1wgi

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[[Image:1wgi.gif|left|200px]]<br />
 
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<applet load="1wgi" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1wgi, resolution 2.20&Aring;" />
 
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'''STRUCTURE OF INORGANIC PYROPHOSPHATASE'''<br />
 
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==Overview==
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==STRUCTURE OF INORGANIC PYROPHOSPHATASE==
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BACKGROUND: Soluble inorganic pyrophosphatase (PPase), an essential enzyme, central to phosphorus metabolism, catalyzes the hydrolysis of the, phosphoanhydride bond in inorganic pyrophosphate. Catalysis requires, divalent metal ions which affect the apparent pKas of the essential, general acid and base on the enzyme, and the pKa of the substrate. Three, to five metal ions are required for maximal activity, depending on pH and, enzyme source. A detailed understanding of catalysis would aid both in, understanding the nature of biological mechanisms of phosphoryl transfer, and in understanding the role of divalent cations. Without a, high-resolution complex structure such a model has previously been, unobtainable. RESULTS: We report the first two high-resolution structures, of yeast PPase, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8994974 (full description)]]
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<StructureSection load='1wgi' size='340' side='right'caption='[[1wgi]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wgi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WGI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wgi OCA], [https://pdbe.org/1wgi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wgi RCSB], [https://www.ebi.ac.uk/pdbsum/1wgi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wgi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IPYR_YEAST IPYR_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wg/1wgi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wgi ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1WGI is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with MN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1]]. Structure known Active Site: . Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WGI OCA]].
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*[[Inorganic pyrophosphatase 3D structures|Inorganic pyrophosphatase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The structural basis for pyrophosphatase catalysis., Heikinheimo P, Lehtonen J, Baykov A, Lahti R, Cooperman BS, Goldman A, Structure. 1996 Dec 15;4(12):1491-508. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8994974 8994974]
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[[Category: Large Structures]]
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[[Category: Inorganic diphosphatase]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Goldman A]]
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[[Category: Goldman, A.]]
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[[Category: Heikinheimo P]]
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[[Category: Heikinheimo, P.]]
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[[Category: MN]]
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[[Category: hydrolase]]
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[[Category: manganese]]
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[[Category: pyrophosphate phosphohydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:31:52 2007''
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Current revision

STRUCTURE OF INORGANIC PYROPHOSPHATASE

PDB ID 1wgi

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