1gsz

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{{STRUCTURE_1gsz| PDB=1gsz | SCENE= }}
 
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===Crystal structure of a squalene cyclase in complex with the potential anticholesteremic drug Ro48-8071===
 
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{{ABSTRACT_PUBMED_12031670}}
 
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==About this Structure==
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==Crystal Structure of a Squalene Cyclase in Complex with the Potential Anticholesteremic Drug Ro48-8071==
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[[1gsz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27009 Atcc 27009]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSZ OCA].
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<StructureSection load='1gsz' size='340' side='right'caption='[[1gsz]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gsz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius Alicyclobacillus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GSZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=R71:[4-({6-[ALLYL(METHYL)AMINO]HEXYL}OXY)-2-FLUOROPHENYL](4-BROMOPHENYL)METHANONE'>R71</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsz OCA], [https://pdbe.org/1gsz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gsz RCSB], [https://www.ebi.ac.uk/pdbsum/1gsz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gsz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SQHC_ALIAD SQHC_ALIAD] Catalyzes the cyclization of squalene into hopene.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/1gsz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gsz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Squalene-hopene cyclase (SHC) catalyzes the conversion of squalene into pentacyclic compounds. It is the prokaryotic counterpart of the eukaryotic oxidosqualene cyclase (OSC) that catalyzes the steroid scaffold formation. Because of clear sequence homology, SHC can serve as a model for OSC, which is an attractive target for anticholesteremic drugs. We have established the crystal structure of SHC complexed with Ro48-8071, a potent inhibitor of OSC and therefore of cholesterol biosynthesis. Ro48-8071 is bound in the active-center cavity of SHC and extends into the channel that connects the cavity with the membrane. The binding site of Ro48-8071 is largely identical with the expected site of squalene; it differs from a previous model based on photoaffinity labeling. The knowledge of the inhibitor binding mode in SHC is likely to help develop more potent inhibitors for OSC.
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==Reference==
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Crystal structure of a squalene cyclase in complex with the potential anticholesteremic drug Ro48-8071.,Lenhart A, Weihofen WA, Pleschke AE, Schulz GE Chem Biol. 2002 May;9(5):639-45. PMID:12031670<ref>PMID:12031670</ref>
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<ref group="xtra">PMID:012031670</ref><references group="xtra"/><references/>
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[[Category: Atcc 27009]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Squalene--hopene cyclase]]
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</div>
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[[Category: Lenhart, A.]]
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<div class="pdbe-citations 1gsz" style="background-color:#fffaf0;"></div>
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[[Category: Pleschke, A E.W.]]
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[[Category: Schulz, G E.]]
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==See Also==
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[[Category: Weihofen, W A.]]
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*[[Squalene-hopene cyclase|Squalene-hopene cyclase]]
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[[Category: Cholesterol biosynthesis]]
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== References ==
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[[Category: Inhibitor oxidosqualene cyclase]]
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<references/>
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[[Category: Isomerase]]
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__TOC__
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[[Category: Monotopic membrane protein]]
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</StructureSection>
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[[Category: Alicyclobacillus acidocaldarius]]
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[[Category: Large Structures]]
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[[Category: Lenhart A]]
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[[Category: Pleschke AEW]]
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[[Category: Schulz GE]]
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[[Category: Weihofen WA]]

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Crystal Structure of a Squalene Cyclase in Complex with the Potential Anticholesteremic Drug Ro48-8071

PDB ID 1gsz

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