3wau

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:02, 8 November 2023) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_3wau| PDB=3wau | SCENE= }}
 
-
===Crystal structure of 4-O-beta-D-mannosyl-D-glucose phosphorylase MGP complexed with M1P===
 
-
{{ABSTRACT_PUBMED_23954514}}
 
-
==Function==
+
==Crystal structure of 4-O-beta-D-mannosyl-D-glucose phosphorylase MGP complexed with M1P==
-
[[http://www.uniprot.org/uniprot/MGP_BACFN MGP_BACFN]] Converts 4-O-beta-D-mannopyranosyl-D-glucopyranose (Man-Glc) to mannose 1-phosphate (Man1P) and glucose. Involved in a mannan catabolic pathway which feeds into glycolysis.<ref>PMID:21539815</ref>
+
<StructureSection load='3wau' size='340' side='right'caption='[[3wau]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3wau]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_fragilis_NCTC_9343 Bacteroides fragilis NCTC 9343]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WAU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WAU FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M1P:ALPHA-D-MANNOSE+1-PHOSPHATE'>M1P</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wau OCA], [https://pdbe.org/3wau PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wau RCSB], [https://www.ebi.ac.uk/pdbsum/3wau PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wau ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MGP_BACFN MGP_BACFN] Converts 4-O-beta-D-mannopyranosyl-D-glucopyranose (Man-Glc) to mannose 1-phosphate (Man1P) and glucose. Involved in a mannan catabolic pathway which feeds into glycolysis.<ref>PMID:21539815</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The crystal structure of a novel component of the mannan biodegradation system, 4-O-beta-d-mannosyl-d-glucose phosphorylase (MGP), was determined to a 1.68-A resolution. The structure of the enzyme revealed a unique homohexameric structure, which was formed by using two helices attached to the N-terminus and C-terminus as a tab for sticking between subunits. The structures of MGP complexes with genuine substrates, 4-O-beta-d-mannosyl-d-glucose and phosphate, and the product d-mannose-1-phosphate were also determined. The complex structures revealed that the invariant residue Asp131, which is supposed to be the general acid/base, did not exist close to the glycosidic Glc-O4 atom, which should be protonated in the catalytic reaction. Also, no solvent molecule that might mediate a proton transfer from Asp131 was observed in the substrate complex structure, suggesting that the catalytic mechanism of MGP is different from those of known disaccharide phosphorylases.
-
==About this Structure==
+
Structure of Novel Enzyme in Mannan Biodegradation Process 4-O-beta-d-Mannosyl-d-Glucose Phosphorylase MGP.,Nakae S, Ito S, Higa M, Senoura T, Wasaki J, Hijikata A, Shionyu M, Ito S, Shirai T J Mol Biol. 2013 Aug 14. pii: S0022-2836(13)00505-6. doi:, 10.1016/j.jmb.2013.08.002. PMID:23954514<ref>PMID:23954514</ref>
-
[[3wau]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacfn Bacfn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WAU OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<ref group="xtra">PMID:023954514</ref><references group="xtra"/><references/>
+
</div>
-
[[Category: 4-O-beta-D-mannosyl-D-glucose phosphorylase]]
+
<div class="pdbe-citations 3wau" style="background-color:#fffaf0;"></div>
-
[[Category: Bacfn]]
+
== References ==
-
[[Category: Higa, M.]]
+
<references/>
-
[[Category: Hijikata, A.]]
+
__TOC__
-
[[Category: Ito, S.]]
+
</StructureSection>
-
[[Category: Nakae, S.]]
+
[[Category: Bacteroides fragilis NCTC 9343]]
-
[[Category: Senoura, T.]]
+
[[Category: Large Structures]]
-
[[Category: Shionyu, M.]]
+
[[Category: Higa M]]
-
[[Category: Shirai, T.]]
+
[[Category: Hijikata A]]
-
[[Category: Wasaki, J.]]
+
[[Category: Ito S]]
-
[[Category: 5-bladed beta propeller fold]]
+
[[Category: Nakae S]]
-
[[Category: Mannan biodegradation]]
+
[[Category: Senoura T]]
-
[[Category: Phosphorylase]]
+
[[Category: Shionyu M]]
-
[[Category: Transferase]]
+
[[Category: Shirai T]]
 +
[[Category: Wasaki J]]

Current revision

Crystal structure of 4-O-beta-D-mannosyl-D-glucose phosphorylase MGP complexed with M1P

PDB ID 3wau

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3wau"
Personal tools