3wkt
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Complex structure of an open form of NADPH-cytochrome P450 reductase and heme oxygenase-1== | |
| + | <StructureSection load='3wkt' size='340' side='right'caption='[[3wkt]], [[Resolution|resolution]] 4.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3wkt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WKT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WKT FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wkt OCA], [https://pdbe.org/3wkt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wkt RCSB], [https://www.ebi.ac.uk/pdbsum/3wkt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wkt ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NCPR_RAT NCPR_RAT] This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | NADPH-cytochrome P450 oxidoreductase (CPR) supplies electrons to various heme proteins including heme oxygenase (HO), which is a key enzyme for heme degradation. Electrons from NADPH flow first to flavin adenine dinucleotide, then to flavin mononucleotide (FMN), and finally to heme in the redox partner. For electron transfer from CPR to its redox partner, the ''closed-open transition'' of CPR is indispensable. Here, we demonstrate that a hinge-shortened CPR variant, which favors an open conformation, makes a stable complex with heme-HO-1 and can support the HO reaction, although its efficiency is extremely limited. Furthermore, we determined the crystal structure of the CPR variant in complex with heme-HO-1 at 4.3-A resolution. The crystal structure of a complex of CPR and its redox partner was previously unidentified. The distance between heme and FMN in this complex (6 A) implies direct electron transfer from FMN to heme. | ||
| - | + | Structural basis for the electron transfer from an open form of NADPH-cytochrome P450 oxidoreductase to heme oxygenase.,Sugishima M, Sato H, Higashimoto Y, Harada J, Wada K, Fukuyama K, Noguchi M Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2524-9. doi:, 10.1073/pnas.1322034111. Epub 2014 Feb 3. PMID:24550278<ref>PMID:24550278</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 3wkt" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Heme oxygenase 3D structures|Heme oxygenase 3D structures]] | ||
| + | *[[NADPH-Cytochrome P450 Reductase|NADPH-Cytochrome P450 Reductase]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Rattus norvegicus]] | ||
| + | [[Category: Fukuyama K]] | ||
| + | [[Category: Harada J]] | ||
| + | [[Category: Higashimoto Y]] | ||
| + | [[Category: Noguchi M]] | ||
| + | [[Category: Sato H]] | ||
| + | [[Category: Sugishima M]] | ||
| + | [[Category: Wada K]] | ||
Current revision
Complex structure of an open form of NADPH-cytochrome P450 reductase and heme oxygenase-1
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