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| - | [[Image:2qbg.gif|left|200px]]<br /><applet load="2qbg" size="350" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[4v54]] This PDB entry is obsolete and replaced by 4v54 |
| - | caption="2qbg, resolution 3.3Å" />
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| - | '''Crystal structure of the bacterial ribosome from Escherichia coli in complex with ribosome recycling factor (RRF). This file contains the 50S subunit of the second 70S ribosome, with RRF bound. The entire crystal structure contains two 70S ribosomes and is described in remark 400.'''<br />
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| - | ==Overview==
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| - | Aminoglycosides are widely used antibiotics that cause messenger RNA decoding errors, block mRNA and transfer RNA translocation, and inhibit ribosome recycling. Ribosome recycling follows the termination of protein synthesis and is aided by ribosome recycling factor (RRF) in bacteria. The molecular mechanism by which aminoglycosides inhibit ribosome recycling is unknown. Here we show in X-ray crystal structures of the Escherichia coli 70S ribosome that RRF binding causes RNA helix H69 of the large ribosomal subunit, which is crucial for subunit association, to swing away from the subunit interface. Aminoglycosides bind to H69 and completely restore the contacts between ribosomal subunits that are disrupted by RRF. These results provide a structural explanation for aminoglycoside inhibition of ribosome recycling.
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| - | ==About this Structure==
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| - | 2QBG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QBG OCA].
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| - | ==Reference==
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| - | Structural basis for aminoglycoside inhibition of bacterial ribosome recycling., Borovinskaya MA, Pai RD, Zhang W, Schuwirth BS, Holton JM, Hirokawa G, Kaji H, Kaji A, Cate JH, Nat Struct Mol Biol. 2007 Aug;14(8):727-32. Epub 2007 Jul 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17660832 17660832]
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| - | [[Category: Escherichia coli]]
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| - | [[Category: Protein complex]]
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| - | [[Category: Thermus thermophilus]]
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| - | [[Category: Borovinskaya, M A.]]
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| - | [[Category: Cate, J H.D.]]
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| - | [[Category: Hirokawa, G.]]
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| - | [[Category: Holton, J M.]]
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| - | [[Category: Kaji, A.]]
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| - | [[Category: Kaji, H.]]
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| - | [[Category: Pai, R D.]]
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| - | [[Category: Schuwirth, B S.]]
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| - | [[Category: Zhang, W.]]
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| - | [[Category: MG]]
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| - | [[Category: ZN]]
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| - | [[Category: ribosome]]
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| - | [[Category: rna-protein complex]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:37:51 2008''
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