2qi2

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[[Image:2qi2.jpg|left|200px]]<br /><applet load="2qi2" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2qi2, resolution 2.90&Aring;" />
 
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'''Crystal structure of the Thermoplasma acidophilum Pelota protein'''<br />
 
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==Overview==
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==Crystal structure of the Thermoplasma acidophilum Pelota protein==
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The yeast protein Dom34 is a key component of no-go decay, by which mRNAs with translational stalls are endonucleolytically cleaved and subsequently degraded. However, the identity of the endoribonuclease is unknown. Homologs of Dom34, called Pelota, are broadly conserved in eukaryotes and archaea. To gain insights into the structure and function of Dom34/Pelota, we have determined the structure of Pelota from Thermoplasma acidophilum (Ta Pelota) and investigated the ribonuclease activity of Dom34/Pelota. The structure of Ta Pelota is tripartite, and its domain 1 has the RNA-binding Sm fold. We have discovered that Ta Pelota has a ribonuclease activity and that its domain 1 is sufficient for the catalytic activity. We also demonstrate that domain 1 of Dom34 has an endoribonuclease activity against defined RNA substrates containing a stem loop, which supports a direct catalytic role of yeast Dom34 in no-go mRNA decay.
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<StructureSection load='2qi2' size='340' side='right'caption='[[2qi2]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2qi2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QI2 FirstGlance]. <br>
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2QI2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QI2 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qi2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qi2 OCA], [https://pdbe.org/2qi2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qi2 RCSB], [https://www.ebi.ac.uk/pdbsum/2qi2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qi2 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structural and functional insights into Dom34, a key component of no-go mRNA decay., Lee HH, Kim YS, Kim KH, Heo I, Kim SK, Kim O, Kim HK, Yoon JY, Kim HS, Kim do J, Lee SJ, Yoon HJ, Kim SJ, Lee BG, Song HK, Kim VN, Park CM, Suh SW, Mol Cell. 2007 Sep 21;27(6):938-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17889667 17889667]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/PELO_THEAC PELO_THEAC] May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity.<ref>PMID:17889667</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qi/2qi2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qi2 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermoplasma acidophilum]]
[[Category: Thermoplasma acidophilum]]
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[[Category: Heo, I H.]]
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[[Category: Heo IH]]
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[[Category: Kim, K H.]]
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[[Category: Kim KH]]
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[[Category: Kim, O.]]
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[[Category: Kim O]]
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[[Category: Kim, S K.]]
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[[Category: Kim SK]]
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[[Category: Kim, Y S.]]
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[[Category: Kim YS]]
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[[Category: Lee, H H.]]
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[[Category: Lee HH]]
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[[Category: Suh, S W.]]
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[[Category: Suh SW]]
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[[Category: cell cycle]]
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[[Category: dom34]]
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[[Category: pelota]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:39:29 2008''
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Current revision

Crystal structure of the Thermoplasma acidophilum Pelota protein

PDB ID 2qi2

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