4nnu

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(New page: '''Unreleased structure''' The entry 4nnu is ON HOLD Authors: Ngo, H.B., Lovely, G.A., Phillips, R., Chan, D.C. Description: Distinct structural features of TFAM drive mitochondrial DN...)
Current revision (17:00, 20 September 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 4nnu is ON HOLD
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==Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation==
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<StructureSection load='4nnu' size='340' side='right'caption='[[4nnu]], [[Resolution|resolution]] 2.81&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4nnu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NNU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.81&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nnu OCA], [https://pdbe.org/4nnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nnu RCSB], [https://www.ebi.ac.uk/pdbsum/4nnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nnu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TFAM_HUMAN TFAM_HUMAN] Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.<ref>PMID:1737790</ref> <ref>PMID:20410300</ref> <ref>PMID:19304746</ref> <ref>PMID:22037172</ref> <ref>PMID:22037171</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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TFAM (transcription factor A, mitochondrial) is a DNA-binding protein that activates transcription at the two major promoters of mitochondrial DNA (mtDNA)-the light strand promoter (LSP) and the heavy strand promoter 1 (HSP1). Equally important, it coats and packages the mitochondrial genome. TFAM has been shown to impose a U-turn on LSP DNA; however, whether this distortion is relevant at other sites is unknown. Here we present crystal structures of TFAM bound to HSP1 and to nonspecific DNA. In both, TFAM similarly distorts the DNA into a U-turn. Yet, TFAM binds to HSP1 in the opposite orientation from LSP explaining why transcription from LSP requires DNA bending, whereas transcription at HSP1 does not. Moreover, the crystal structures reveal dimerization of DNA-bound TFAM. This dimerization is dispensable for DNA bending and transcriptional activation but is important in DNA compaction. We propose that TFAM dimerization enhances mitochondrial DNA compaction by promoting looping of the DNA.
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Authors: Ngo, H.B., Lovely, G.A., Phillips, R., Chan, D.C.
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Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation.,Ngo HB, Lovely GA, Phillips R, Chan DC Nat Commun. 2014 Jan 17;5:3077. doi: 10.1038/ncomms4077. PMID:24435062<ref>PMID:24435062</ref>
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Description: Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4nnu" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Chan DC]]
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[[Category: Lovely GA]]
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[[Category: Ngo HB]]
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[[Category: Phillips R]]

Current revision

Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation

PDB ID 4nnu

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