4nov
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4nov is ON HOLD Authors: Till, M., Arcus, V.L. Description: Xsa43E, a GH43 family enzyme from Butyrivibrio proteoclasticus) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Xsa43E, a GH43 family enzyme from Butyrivibrio proteoclasticus== | |
| + | <StructureSection load='4nov' size='340' side='right'caption='[[4nov]], [[Resolution|resolution]] 1.33Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4nov]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Butyrivibrio_proteoclasticus_B316 Butyrivibrio proteoclasticus B316]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NOV FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.33Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nov OCA], [https://pdbe.org/4nov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nov RCSB], [https://www.ebi.ac.uk/pdbsum/4nov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nov ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The rumen of dairy cattle can be thought of as a large, stable fermentation vat and as such it houses a large and diverse community of microorganisms. The bacterium Butyrivibrio proteoclasticus is a representative of a significant component of this microbial community. It is a xylan-degrading organism whose genome encodes a large number of open reading frames annotated as fibre-degrading enzymes. This suite of enzymes is essential for the organism to utilize the plant material within the rumen as a fuel source, facilitating its survival in this competitive environment. Xsa43E, a GH43 enzyme from B. proteoclasticus, has been structurally and functionally characterized. Here, the structure of selenomethionine-derived Xsa43E determined to 1.3 A resolution using single-wavelength anomalous diffraction is reported. Xsa43E possesses the characteristic five-bladed beta-propeller domain seen in all GH43 enzymes. GH43 enzymes can have a range of functions, and the functional characterization of Xsa43E shows it to be an arabinofuranosidase capable of cleaving arabinose side chains from short segments of xylan. Full functional and structural characterization of xylan-degrading enzymes will aid in creating an enzyme cocktail that can be used to completely degrade plant material into simple sugars. These molecules have a range of applications as starting materials for many industrial processes, including renewable alternatives to fossil fuels. | ||
| - | + | Structural analysis of the GH43 enzyme Xsa43E from Butyrivibrio proteoclasticus.,Till M, Goldstone D, Card G, Attwood GT, Moon CD, Arcus VL Acta Crystallogr F Struct Biol Commun. 2014 Sep 1;70(Pt 9):1193-8. doi:, 10.1107/S2053230X14014745. Epub 2014 Aug 29. PMID:25195890<ref>PMID:25195890</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4nov" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Butyrivibrio proteoclasticus B316]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Arcus VL]] | ||
| + | [[Category: Till M]] | ||
Current revision
Xsa43E, a GH43 family enzyme from Butyrivibrio proteoclasticus
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