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2qom

From Proteopedia

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[[Image:2qom.jpg|left|200px]]<br /><applet load="2qom" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2qom, resolution 2.66&Aring;" />
 
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'''The crystal structure of the E.coli EspP autotransporter Beta-domain.'''<br />
 
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==Overview==
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==The crystal structure of the E.coli EspP autotransporter Beta-domain.==
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Autotransporters are virulence factors produced by Gram-negative bacteria. They consist of two domains, an N-terminal 'passenger' domain and a C-terminal beta-domain. beta-domains form beta-barrel structures in the outer membrane while passenger domains are translocated into the extracellular space. In some autotransporters, the two domains are separated by proteolytic cleavage. Using X-ray crystallography, we solved the 2.7-A structure of the post-cleavage state of the beta-domain of EspP, an autotransporter produced by Escherichia coli strain O157:H7. The structure consists of a 12-stranded beta-barrel with the passenger domain-beta-domain cleavage junction located inside the barrel pore, approximately midway between the extracellular and periplasmic surfaces of the outer membrane. The structure reveals an unprecedented intra-barrel cleavage mechanism and suggests that two conformational changes occur in the beta-domain after cleavage, one conferring increased stability on the beta-domain and another restricting access to the barrel pore.
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<StructureSection load='2qom' size='340' side='right'caption='[[2qom]], [[Resolution|resolution]] 2.66&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2qom]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QOM FirstGlance]. <br>
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2QOM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOM OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.66&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qom OCA], [https://pdbe.org/2qom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qom RCSB], [https://www.ebi.ac.uk/pdbsum/2qom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qom ProSAT]</span></td></tr>
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==Reference==
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</table>
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Autotransporter structure reveals intra-barrel cleavage followed by conformational changes., Barnard TJ, Dautin N, Lukacik P, Bernstein HD, Buchanan SK, Nat Struct Mol Biol. 2007 Dec;14(12):1214-20. Epub 2007 Nov 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17994105 17994105]
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== Function ==
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[[Category: Escherichia coli]]
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[https://www.uniprot.org/uniprot/ESPP_ECO57 ESPP_ECO57] Serine protease capable of cleaving pepsin A and human coagulation factor V, which may contribute to the mucosal hemorrhage observed in hemorrhagic colitis.<ref>PMID:9194704</ref> <ref>PMID:15615856</ref>
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Barnard, T J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Bernstein, H D.]]
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Check<jmol>
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[[Category: Buchanan, S K.]]
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<jmolCheckbox>
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[[Category: Dautin, N.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qo/2qom_consurf.spt"</scriptWhenChecked>
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[[Category: Lukacik, P.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: autotransporter]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: beta-barrel]]
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</jmolCheckbox>
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[[Category: beta-domain]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qom ConSurf].
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[[Category: hydrolase]]
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<div style="clear:both"></div>
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[[Category: outer membrane protein]]
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== References ==
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[[Category: plasmid]]
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<references/>
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[[Category: protease]]
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__TOC__
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[[Category: secreted]]
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</StructureSection>
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[[Category: serine protease]]
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[[Category: Escherichia coli O157:H7]]
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[[Category: transmembrane]]
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[[Category: Large Structures]]
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[[Category: virulence]]
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[[Category: Barnard TJ]]
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[[Category: zymogen]]
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[[Category: Bernstein HD]]
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[[Category: Buchanan SK]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:41:04 2008''
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[[Category: Dautin N]]
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[[Category: Lukacik P]]

Current revision

The crystal structure of the E.coli EspP autotransporter Beta-domain.

PDB ID 2qom

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