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| - | [[Image:2qua.jpg|left|200px]]<br /><applet load="2qua" size="350" color="white" frame="true" align="right" spinBox="true" | |
| - | caption="2qua, resolution 1.95Å" /> | |
| - | '''Crystal structure of LipA from Serratia marcescens'''<br /> | |
| | | | |
| - | ==Overview== | + | ==Crystal structure of LipA from Serratia marcescens== |
| - | Lipase LipA from Serratia marcescens is a 613-amino acid enzyme belonging to family I.3 of lipolytic enzymes that has an important biotechnological application in the production of a chiral precursor for the coronary vasodilator diltiazem. Like other family I.3 lipases, LipA is secreted by Gram-negative bacteria via a type I secretion system and possesses 13 copies of a calcium binding tandem repeat motif, GGXGXDXUX (U, hydrophobic amino acids), in the C-terminal part of the polypeptide chain. The 1.8-A crystal structure of LipA reveals a close relation to eukaryotic lipases, whereas family I.1 and I.2 enzymes appear to be more distantly related. Interestingly, the structure shows for the N-terminal lipase domain a variation on the canonical alpha/beta hydrolase fold in an open conformation, where the putative lid helix is anchored by a Ca(2+) ion essential for activity. Another novel feature observed in this lipase structure is the presence of a helical hairpin additional to the putative lid helix that exposes a hydrophobic surface to the aqueous medium and might function as an additional lid. The tandem repeats form two separated parallel beta-roll domains that pack tightly against each other. Variations of the consensus sequence of the tandem repeats within the second beta-roll result in an asymmetric Ca(2+) binding on only one side of the roll. The analysis of the properties of the beta-roll domains suggests an intramolecular chaperone function.
| + | <StructureSection load='2qua' size='340' side='right'caption='[[2qua]], [[Resolution|resolution]] 1.95Å' scene=''> |
| | + | == Structural highlights == |
| | + | <table><tr><td colspan='2'>[[2qua]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QUA FirstGlance]. <br> |
| | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> |
| | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qua OCA], [https://pdbe.org/2qua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qua RCSB], [https://www.ebi.ac.uk/pdbsum/2qua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qua ProSAT]</span></td></tr> |
| | + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q59933_SERMA Q59933_SERMA] |
| | + | == Evolutionary Conservation == |
| | + | [[Image:Consurf_key_small.gif|200px|right]] |
| | + | Check<jmol> |
| | + | <jmolCheckbox> |
| | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qu/2qua_consurf.spt"</scriptWhenChecked> |
| | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | + | <text>to colour the structure by Evolutionary Conservation</text> |
| | + | </jmolCheckbox> |
| | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qua ConSurf]. |
| | + | <div style="clear:both"></div> |
| | | | |
| - | ==About this Structure== | + | ==See Also== |
| - | 2QUA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Known structural/functional Sites: <scene name='pdbsite=AC1:Ca+Binding+Site+For+Residue+A+614'>AC1</scene>, <scene name='pdbsite=AC2:Ca+Binding+Site+For+Residue+A+615'>AC2</scene>, <scene name='pdbsite=AC3:Ca+Binding+Site+For+Residue+A+616'>AC3</scene>, <scene name='pdbsite=AC4:Ca+Binding+Site+For+Residue+A+617'>AC4</scene>, <scene name='pdbsite=AC5:Ca+Binding+Site+For+Residue+A+618'>AC5</scene>, <scene name='pdbsite=AC6:Ca+Binding+Site+For+Residue+A+619'>AC6</scene>, <scene name='pdbsite=AC7:Ca+Binding+Site+For+Residue+A+620'>AC7</scene> and <scene name='pdbsite=AC8:Ca+Binding+Site+For+Residue+A+621'>AC8</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QUA OCA].
| + | *[[Lipase 3D Structures|Lipase 3D Structures]] |
| - | | + | __TOC__ |
| - | ==Reference==
| + | </StructureSection> |
| - | A calcium-gated lid and a large beta-roll sandwich are revealed by the crystal structure of extracellular lipase from Serratia marcescens., Meier R, Drepper T, Svensson V, Jaeger KE, Baumann U, J Biol Chem. 2007 Oct 26;282(43):31477-83. Epub 2007 Aug 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17728256 17728256]
| + | [[Category: Large Structures]] |
| | [[Category: Serratia marcescens]] | | [[Category: Serratia marcescens]] |
| - | [[Category: Single protein]]
| + | [[Category: Baumann U]] |
| - | [[Category: Triacylglycerol lipase]]
| + | [[Category: Meier R]] |
| - | [[Category: Baumann, U.]] | + | |
| - | [[Category: Meier, R.]] | + | |
| - | [[Category: CA]]
| + | |
| - | [[Category: alpha/beta hydrolase]]
| + | |
| - | [[Category: beta roll]]
| + | |
| - | [[Category: helical hairpin]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:42:27 2008''
| + | |
