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2afn

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[[Image:2afn.gif|left|200px]]<br />
 
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<applet load="2afn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2afn, resolution 2.0&Aring;" />
 
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'''STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE AND A COPPER SITE MUTANT, M150E, THAT CONTAINS ZINC'''<br />
 
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==Overview==
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==STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE AND A COPPER SITE MUTANT, M150E, THAT CONTAINS ZINC==
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The structures at 2.0 and 2.25 A resolution of native and recombinant, nitrite reductase from Alcaligenes faecalis show that they are identical, to each other and very similar to nitrite reductase from Achromobacter, cycloclastes. The crystallographic structure of a mutant, M150E, which, unlike the wild-type protein cannot be reduced by pseudoazurin, shows that, the glutamate replacement for methionine binds to a metal at the type I Cu, site via only one oxygen. Anomalous scattering data collected at, wavelengths of 1.040 and 1.377 A reveal that the metal at the type I site, is a Zn. No significant differences from the native structure other than, local perturbations at the type I site are seen. A local pseudo 2-fold, axis relates the two domains of different monomers which form the ... [[http://ispc.weizmann.ac.il/pmbin/getpm?7547950 (full description)]]
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<StructureSection load='2afn' size='340' side='right'caption='[[2afn]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2afn]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1afn 1afn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AFN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2afn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2afn OCA], [https://pdbe.org/2afn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2afn RCSB], [https://www.ebi.ac.uk/pdbsum/2afn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2afn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NIR_ALCFA NIR_ALCFA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/2afn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2afn ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2AFN is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]] with CU as [[http://en.wikipedia.org/wiki/ligand ligand]]. This structure superseeds the now removed PDB entry 1AFN. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_1.7.2.1 Transferred entry: 1.7.2.1]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.3 1.7.99.3]]. Structure known Active Sites: CU1, CU2, CU3, CU4, CU5 and CU6. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AFN OCA]].
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*[[Nitrite reductase 3D structures|Nitrite reductase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of Alcaligenes faecalis nitrite reductase and a copper site mutant, M150E, that contains zinc., Murphy ME, Turley S, Kukimoto M, Nishiyama M, Horinouchi S, Sasaki H, Tanokura M, Adman ET, Biochemistry. 1995 Sep 26;34(38):12107-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7547950 7547950]
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[[Category: Alcaligenes faecalis]]
[[Category: Alcaligenes faecalis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Transferred entry: 1.7.2.1]]
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[[Category: Adman ET]]
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[[Category: Adman, E.T.]]
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[[Category: Murphy MEP]]
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[[Category: Murphy, M.E.P.]]
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[[Category: Turley S]]
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[[Category: Turley, S.]]
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[[Category: CU]]
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[[Category: (nitric oxide (a))]]
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[[Category: copper]]
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[[Category: fad]]
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[[Category: flavoprotein]]
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[[Category: nitrite assimilation]]
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[[Category: oxidoreductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:34:26 2007''
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Current revision

STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE AND A COPPER SITE MUTANT, M150E, THAT CONTAINS ZINC

PDB ID 2afn

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