2r5a

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[[Image:2r5a.gif|left|200px]]<br /><applet load="2r5a" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2r5a, resolution 2.30&Aring;" />
 
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'''Crystal Structure of the two MBT repeats from Sex-Comb on Midleg (SCM) in complex with methyl lysine'''<br />
 
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==Overview==
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==Crystal Structure of the two MBT repeats from Sex-Comb on Midleg (SCM) in complex with methyl lysine==
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Sex comb on midleg (Scm) is a member of the Polycomb group of proteins involved in the maintenance of repression of Hox and other developmental control genes in Drosophila. The two malignant brain tumour (MBT) repeats of Scm form a domain that preferentially binds to monomethylated lysine residues either as a free amino acid or in the context of peptides, while unmodified or di- or trimethylated lysine residues are bound with significantly lower affinity. The crystal structure of a monomethyl-lysine-containing histone tail peptide bound to the MBT repeat domain shows that the methyl-lysine side chain occupies a binding pocket in the second MBT repeat formed by three conserved aromatic residues and one aspartate. Insertion of the monomethylated side chain into this pocket seems to be the main contributor to the binding affinity. Functional analyses in Drosophila show that the MBT domain of Scm and its methyl-lysine-binding activity are required for repression of Hox genes.
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<StructureSection load='2r5a' size='340' side='right'caption='[[2r5a]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2r5a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R5A FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLZ:N-METHYL-LYSINE'>MLZ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r5a OCA], [https://pdbe.org/2r5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r5a RCSB], [https://www.ebi.ac.uk/pdbsum/2r5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r5a ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SCM_DROME SCM_DROME] Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility.<ref>PMID:15280237</ref> [UniProtKB:Q9W3C1]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r5/2r5a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r5a ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2R5A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=MLZ:'>MLZ</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Mlz+Binding+Site+For+Residue+A+436'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R5A OCA].
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*[[Polycomb complex proteins 3D structures|Polycomb complex proteins 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structural and functional analyses of methyl-lysine binding by the malignant brain tumour repeat protein Sex comb on midleg., Grimm C, de Ayala Alonso AG, Rybin V, Steuerwald U, Ly-Hartig N, Fischle W, Muller J, Muller CW, EMBO Rep. 2007 Nov;8(11):1031-7. Epub 2007 Oct 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17932512 17932512]
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Grimm, C.]]
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[[Category: Grimm C]]
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[[Category: Mueller, C W.]]
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[[Category: Mueller CW]]
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[[Category: Steuerwald, U.]]
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[[Category: Steuerwald U]]
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[[Category: MLZ]]
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[[Category: chromatin regulator]]
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[[Category: developmental protein]]
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[[Category: mbt]]
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[[Category: mbt repeat]]
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[[Category: metal-binding]]
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[[Category: nucleus]]
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[[Category: polycomb]]
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[[Category: repressor]]
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[[Category: scm]]
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[[Category: sex comb on midleg]]
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[[Category: transcription]]
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[[Category: transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:44:44 2008''
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Current revision

Crystal Structure of the two MBT repeats from Sex-Comb on Midleg (SCM) in complex with methyl lysine

PDB ID 2r5a

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