2r9q

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[[Image:2r9q.jpg|left|200px]]<br /><applet load="2r9q" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2r9q, resolution 2.20&Aring;" />
 
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'''Crystal structure of 2'-deoxycytidine 5'-triphosphate deaminase from Agrobacterium tumefaciens'''<br />
 
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==Overview==
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==Crystal structure of 2'-deoxycytidine 5'-triphosphate deaminase from Agrobacterium tumefaciens==
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We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
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<StructureSection load='2r9q' size='340' side='right'caption='[[2r9q]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2r9q]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_fabrum_str._C58 Agrobacterium fabrum str. C58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R9Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R9Q FirstGlance]. <br>
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2R9Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens_str._c58 Agrobacterium tumefaciens str. c58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R9Q OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r9q OCA], [https://pdbe.org/2r9q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r9q RCSB], [https://www.ebi.ac.uk/pdbsum/2r9q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r9q ProSAT], [https://www.topsan.org/Proteins/MCSG/2r9q TOPSAN]</span></td></tr>
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==Reference==
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</table>
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In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17982461 17982461]
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== Function ==
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[[Category: Agrobacterium tumefaciens str. c58]]
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[https://www.uniprot.org/uniprot/A9CKA1_AGRFC A9CKA1_AGRFC]
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Dong, A.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Edwards, A M.]]
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Check<jmol>
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[[Category: Joachimiak, A.]]
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<jmolCheckbox>
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r9/2r9q_consurf.spt"</scriptWhenChecked>
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[[Category: Savchenko, A.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Xu, X.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Zhang, R.]]
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</jmolCheckbox>
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[[Category: 2'-deoxycytidine 5'-triphosphate deaminase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r9q ConSurf].
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[[Category: hydrolase]]
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<div style="clear:both"></div>
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[[Category: mcsg]]
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__TOC__
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[[Category: midwest center for structural genomics]]
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</StructureSection>
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[[Category: protein structure initiative]]
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[[Category: Agrobacterium fabrum str. C58]]
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[[Category: psi-2]]
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[[Category: Large Structures]]
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[[Category: structural genomics]]
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[[Category: Dong A]]
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[[Category: Edwards AM]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:45:40 2008''
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[[Category: Joachimiak A]]
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[[Category: Savchenko A]]
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[[Category: Xu X]]
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[[Category: Zhang R]]

Current revision

Crystal structure of 2'-deoxycytidine 5'-triphosphate deaminase from Agrobacterium tumefaciens

PDB ID 2r9q

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