4m8o

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{{STRUCTURE_4m8o| PDB=4m8o | SCENE= }}
 
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===TERNARY COMPLEX OF DNA POLYMERASE EPSILON WITH AN INCOMING dATP===
 
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==Function==
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==TERNARY COMPLEX OF DNA POLYMERASE EPSILON WITH AN INCOMING dATP==
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[[http://www.uniprot.org/uniprot/DPOE_YEAST DPOE_YEAST]] DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.<ref>PMID:12124389</ref>
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<StructureSection load='4m8o' size='340' side='right'caption='[[4m8o]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4m8o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M8O FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5IU:5-IODO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>5IU</scene>, <scene name='pdbligand=C38:5-IODO-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>C38</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene>, <scene name='pdbligand=DTP:2-DEOXYADENOSINE+5-TRIPHOSPHATE'>DTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TAU:2-AMINOETHANESULFONIC+ACID'>TAU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m8o OCA], [https://pdbe.org/4m8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m8o RCSB], [https://www.ebi.ac.uk/pdbsum/4m8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m8o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DPOE_YEAST DPOE_YEAST] DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.<ref>PMID:12124389</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DNA polymerase varepsilon (Pol varepsilon) is a high-fidelity polymerase that has been shown to participate in leading-strand synthesis during DNA replication in eukaryotic cells. We present here a ternary structure of the catalytic core of Pol varepsilon (142 kDa) from Saccharomyces cerevisiae in complex with DNA and an incoming nucleotide. This structure provides information about the selection of the correct nucleotide and the positions of amino acids that might be critical for proofreading activity. Pol varepsilon has the highest fidelity among B-family polymerases despite the absence of an extended beta-hairpin loop that is required for high-fidelity replication by other B-family polymerases. Moreover, the catalytic core has a new domain that allows Pol varepsilon to encircle the nascent double-stranded DNA. Altogether, the structure provides an explanation for the high processivity and high fidelity of leading-strand DNA synthesis in eukaryotes.
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==About this Structure==
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Structural basis for processive DNA synthesis by yeast DNA polymerase varepsilon.,Hogg M, Osterman P, Bylund GO, Ganai RA, Lundstrom EB, Sauer-Eriksson AE, Johansson E Nat Struct Mol Biol. 2014 Jan;21(1):49-55. doi: 10.1038/nsmb.2712. Epub 2013 Dec , 1. PMID:24292646<ref>PMID:24292646</ref>
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[[4m8o]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M8O OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<references group="xtra"/><references/>
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</div>
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[[Category: Baker's yeast]]
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<div class="pdbe-citations 4m8o" style="background-color:#fffaf0;"></div>
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[[Category: DNA-directed DNA polymerase]]
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[[Category: Hogg, M.]]
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==See Also==
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[[Category: Johansson, E.]]
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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[[Category: Osterman, P.]]
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== References ==
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[[Category: Sauer-Eriksson, A E.]]
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<references/>
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[[Category: Dna polymerase b type]]
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__TOC__
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[[Category: Dna replication]]
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</StructureSection>
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[[Category: Dna synthesis]]
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[[Category: Large Structures]]
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[[Category: Metal-binding]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Nucleotidyltransferase]]
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[[Category: Hogg M]]
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[[Category: Protein-dna complex]]
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[[Category: Johansson E]]
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[[Category: Transferase dna complex]]
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[[Category: Osterman P]]
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[[Category: Transferase-dna complex]]
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[[Category: Sauer-Eriksson AE]]

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TERNARY COMPLEX OF DNA POLYMERASE EPSILON WITH AN INCOMING dATP

PDB ID 4m8o

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