2reb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:21, 21 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2reb.gif|left|200px]]<br /><applet load="2reb" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2reb, resolution 2.3&Aring;" />
 
-
'''THE STRUCTURE OF THE E. COLI RECA PROTEIN MONOMER AND POLYMER'''<br />
 
-
==Overview==
+
==THE STRUCTURE OF THE E. COLI RECA PROTEIN MONOMER AND POLYMER==
-
The crystal structure of the recA protein from Escherichia coli at 2.3-A resolution reveals a major domain that binds ADP and probably single- and double-stranded DNA. Two smaller subdomains at the N and C termini protrude from the protein and respectively stabilize a 6(1) helical polymer of protein subunits and interpolymer bundles. This polymer structure closely resembles that of recA/DNA filaments determined by electron microscopy. Mutations in recA protein that enhance coprotease, DNA-binding and/or strand-exchange activity can be explained if the interpolymer interactions in the crystal reflect a regulatory mechanism in vivo.
+
<StructureSection load='2reb' size='340' side='right'caption='[[2reb]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2reb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2REB FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2reb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reb OCA], [https://pdbe.org/2reb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2reb RCSB], [https://www.ebi.ac.uk/pdbsum/2reb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2reb ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RECA_ECOLI RECA_ECOLI] Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.[HAMAP-Rule:MF_00268]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/re/2reb_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2reb ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
2REB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REB OCA].
+
*[[Recombinase A|Recombinase A]]
-
 
+
*[[3D structures of recombinase A|3D structures of recombinase A]]
-
==Reference==
+
__TOC__
-
The structure of the E. coli recA protein monomer and polymer., Story RM, Weber IT, Steitz TA, Nature. 1992 Jan 23;355(6358):318-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1731246 1731246]
+
</StructureSection>
-
[[Category: Deleted entry]]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Steitz, T A.]]
+
[[Category: Steitz TA]]
-
[[Category: Story, R M.]]
+
[[Category: Story RM]]
-
[[Category: homologous recombination]]
+
-
[[Category: self-cleavage stimulation]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:46:45 2008''
+

Current revision

THE STRUCTURE OF THE E. COLI RECA PROTEIN MONOMER AND POLYMER

PDB ID 2reb

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2reb"
Personal tools