4ljy

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{{STRUCTURE_4ljy| PDB=4ljy | SCENE= }}
 
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===Crystal structure of RNA splicing effector Prp5 in complex with ADP===
 
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==Function==
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==Crystal structure of RNA splicing effector Prp5 in complex with ADP==
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[[http://www.uniprot.org/uniprot/PRP5_YEAST PRP5_YEAST]] ATP-dependent RNA helicase involved spliceosome assembly and in nuclear splicing. Catalyzes an ATP-dependent conformational change of U2 snRNP. Bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA.<ref>PMID:8405998</ref> <ref>PMID:8065365</ref> <ref>PMID:8969184</ref> <ref>PMID:8969185</ref> <ref>PMID:8622683</ref> <ref>PMID:11927574</ref> <ref>PMID:14610285</ref> <ref>PMID:16314500</ref>
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<StructureSection load='4ljy' size='340' side='right'caption='[[4ljy]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ljy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LJY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ljy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ljy OCA], [https://pdbe.org/4ljy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ljy RCSB], [https://www.ebi.ac.uk/pdbsum/4ljy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ljy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PRP5_YEAST PRP5_YEAST] ATP-dependent RNA helicase involved spliceosome assembly and in nuclear splicing. Catalyzes an ATP-dependent conformational change of U2 snRNP. Bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA.<ref>PMID:8405998</ref> <ref>PMID:8065365</ref> <ref>PMID:8969184</ref> <ref>PMID:8969185</ref> <ref>PMID:8622683</ref> <ref>PMID:11927574</ref> <ref>PMID:14610285</ref> <ref>PMID:16314500</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The DEAD-box adenosine triphosphatase (ATPase) Prp5p facilitates U2 small nuclear ribonucleoprotein particle (snRNP) binding to the intron branch site region during spliceosome assembly. We present crystal structures of S. cerevisiae Prp5p alone and in complex with ADP at 2.12 A and 1.95 A resolution. The three-dimensional packing of Prp5p subdomains differs strikingly from that so far observed in other DEAD-box proteins: two RecA-like subdomains adopt an "open state" conformation stabilized by extensive interactions involving sequences that flank the two subdomains. This conformation is distinct from that required for ATP hydrolysis. Consistent with this, Prp5p mutations that destabilize interdomain interactions exhibited increased ATPase activity in vitro and inhibited splicing of suboptimal branch site substrates in vivo, whereas restoration of interdomain interactions reversed these effects. We conclude that the Prp5p open state conformation is biologically relevant and that disruption of the interdomain interaction facilitates a large-scale conformational change of Prp5p during U2 snRNP-branch site recognition.
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==About this Structure==
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Crystal structure of Prp5p reveals interdomain interactions that impact spliceosome assembly.,Zhang ZM, Yang F, Zhang J, Tang Q, Li J, Gu J, Zhou J, Xu YZ Cell Rep. 2013 Dec 12;5(5):1269-78. doi: 10.1016/j.celrep.2013.10.047. Epub 2013 , Nov 27. PMID:24290758<ref>PMID:24290758</ref>
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[[4ljy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LJY OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<references group="xtra"/><references/>
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</div>
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[[Category: RNA helicase]]
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<div class="pdbe-citations 4ljy" style="background-color:#fffaf0;"></div>
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[[Category: Li, J.]]
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== References ==
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[[Category: Xu, Y.]]
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<references/>
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[[Category: Yang, F.]]
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__TOC__
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[[Category: Zhang, Z M.]]
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</StructureSection>
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[[Category: Zhou, J.]]
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[[Category: Large Structures]]
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[[Category: Dead box]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Hydrolase]]
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[[Category: Li J]]
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[[Category: Prp5]]
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[[Category: Xu Y]]
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[[Category: Rna splicing]]
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[[Category: Yang F]]
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[[Category: Zhang Z-M]]
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[[Category: Zhou J]]

Current revision

Crystal structure of RNA splicing effector Prp5 in complex with ADP

PDB ID 4ljy

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