4n4o
From Proteopedia
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| - | {{STRUCTURE_4n4o| PDB=4n4o | SCENE= }} | ||
| - | ===Nitrosomonas europea HAO soaked in NH2OH=== | ||
| - | == | + | ==Nitrosomonas europea HAO soaked in NH2OH== |
| - | [[4n4o]] is a 6 chain structure with sequence from [ | + | <StructureSection load='4n4o' size='340' side='right'caption='[[4n4o]], [[Resolution|resolution]] 2.47Å' scene=''> |
| - | [[Category: | + | == Structural highlights == |
| + | <table><tr><td colspan='2'>[[4n4o]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitrosomonas_europaea Nitrosomonas europaea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N4O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N4O FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.472Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=12P:DODECAETHYLENE+GLYCOL'>12P</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HOA:HYDROXYAMINE'>HOA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n4o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n4o OCA], [https://pdbe.org/4n4o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n4o RCSB], [https://www.ebi.ac.uk/pdbsum/4n4o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n4o ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/HAO_NITEU HAO_NITEU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nitric oxide (NO) is an important molecule in all domains of life with significant biological functions in both pro- and eukaryotes. Anaerobic ammonium-oxidizing (anammox) bacteria that contribute substantially to the release of fixed nitrogen into the atmosphere use the oxidizing power of NO to activate inert ammonium into hydrazine (N2H4). Here, we describe an enzyme from the anammox bacterium Kuenenia stuttgartiensis that uses a novel pathway to make NO from hydroxylamine. This new enzyme is related to octaheme hydroxylamine oxidoreductase (HAO), a key protein in aerobic ammonium-oxidizing bacteria. By a multiphasic approach including the determination of the crystal structure of the K. stuttgartiensis enzyme at 1.8Angstrom resolution and refinement and reassessment of the HAO structure from Nitrosomonas europaea, both in the presence and absence of their substrates, we propose a model for NO formation by the K. stuttgartiensis enzyme. Our results expand the understanding of the functions that the widespread family of octaheme proteins have. | ||
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| + | Structural basis of biological NO generation by octaheme oxidoreductases.,Maalcke WJ, Dietl A, Marritt SJ, Butt JN, Jetten MS, Keltjens JT, Barends TR, Kartal B J Biol Chem. 2013 Dec 3. PMID:24302732<ref>PMID:24302732</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4n4o" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Nitrosomonas europaea]] | [[Category: Nitrosomonas europaea]] | ||
| - | [[Category: Barends | + | [[Category: Barends TRMB]] |
| - | [[Category: Butt | + | [[Category: Butt JN]] |
| - | [[Category: Dietl | + | [[Category: Dietl A]] |
| - | [[Category: Jetten | + | [[Category: Jetten MSM]] |
| - | [[Category: Kartal | + | [[Category: Kartal B]] |
| - | [[Category: Keltjens | + | [[Category: Keltjens JT]] |
| - | [[Category: Maalcke | + | [[Category: Maalcke WJ]] |
| - | [[Category: Marritt | + | [[Category: Marritt SJ]] |
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| - | + | ||
Current revision
Nitrosomonas europea HAO soaked in NH2OH
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