4kr1

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{{STRUCTURE_4kr1| PDB=4kr1 | SCENE= }}
 
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===Crystal structure of the kinetechore protein Iml3 from budding yeast===
 
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{{ABSTRACT_PUBMED_24311582}}
 
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==Function==
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==Crystal structure of the kinetechore protein Iml3 from budding yeast==
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[[http://www.uniprot.org/uniprot/IML3_YEAST IML3_YEAST]] Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.<ref>PMID:11361335</ref> <ref>PMID:15241623</ref>
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<StructureSection load='4kr1' size='340' side='right'caption='[[4kr1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4kr1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KR1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kr1 OCA], [https://pdbe.org/4kr1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kr1 RCSB], [https://www.ebi.ac.uk/pdbsum/4kr1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kr1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CENPL_YEAST CENPL_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.<ref>PMID:11361335</ref> <ref>PMID:15241623</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human CENP-N and CENP-L have been reported to selectively recognize the CENP-A nucleosome and to contribute to recruiting other constitutive centromere-associated network (CCAN) complexes involved in assembly of the inner kinetochore. As their homologues, Chl4 and Iml3 from budding yeast function in a similar way in de novo assembly of the kinetochore. A lack of biochemical and structural information precludes further understanding of their exact role at the molecular level. Here, the crystal structure of Iml3 is presented and the structure shows that Iml3 adopts an elongated conformation with a series of intramolecular interactions. Pull-down assays revealed that the C-terminal domain of Chl4, which forms a dimer in solution, is responsible for Iml3 binding. Acting as a heterodimer, the Chl4-Iml3 complex exhibits a low-affinity nonspecific DNA-binding activity which may play an important role in the kinetochore-assembly process.
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==About this Structure==
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Structural insights into the role of the Chl4-Iml3 complex in kinetochore assembly.,Guo Q, Tao Y, Liu H, Teng M, Li X Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2412-9. doi:, 10.1107/S0907444913022397. Epub 2013 Nov 19. PMID:24311582<ref>PMID:24311582</ref>
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[[4kr1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KR1 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024311582</ref><references group="xtra"/><references/>
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</div>
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[[Category: Guo, Q.]]
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<div class="pdbe-citations 4kr1" style="background-color:#fffaf0;"></div>
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[[Category: Tao, Y.]]
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== References ==
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[[Category: Teng, M.]]
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<references/>
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[[Category: Cell cycle]]
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__TOC__
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[[Category: Chromosome segregation]]
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</StructureSection>
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[[Category: Kinetochore protein]]
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[[Category: Large Structures]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Guo Q]]
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[[Category: Tao Y]]
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[[Category: Teng M]]

Current revision

Crystal structure of the kinetechore protein Iml3 from budding yeast

PDB ID 4kr1

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