2m49

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{{STRUCTURE_2m49| PDB=2m49 | SCENE= }}
 
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===Structural Insights into Human S100B and Basic Fibroblast Growth Factor (FGF2) Interaction===
 
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{{ABSTRACT_PUBMED_24063890}}
 
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==Function==
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==Structural Insights into Human S100B and Basic Fibroblast Growth Factor (FGF2) Interaction==
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[[http://www.uniprot.org/uniprot/FGF2_HUMAN FGF2_HUMAN]] Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.<ref>PMID:1721615</ref> <ref>PMID:8663044</ref> [[http://www.uniprot.org/uniprot/S100B_HUMAN S100B_HUMAN]] Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization.<ref>PMID:20351179</ref>
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<StructureSection load='2m49' size='340' side='right'caption='[[2m49]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2m49]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M49 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M49 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m49 OCA], [https://pdbe.org/2m49 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m49 RCSB], [https://www.ebi.ac.uk/pdbsum/2m49 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m49 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FGF2_HUMAN FGF2_HUMAN] Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.<ref>PMID:1721615</ref> <ref>PMID:8663044</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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S100B is a calcium sensing protein belonging to the S100 protein family with intracellular and extracellular roles. It is one of the EF hand homodimeric proteins, which is known to interact with various protein targets to regulate varied biological functions. Extracellular S100B has been recently reported to interact with FGF2 in a RAGE-independent manner. However, the recognition mechanism of S100B-FGF2 interaction at the molecular level remains unclear. In this study, the critical residues on S100B-FGF2 interface were mapped by combined information derived from NMR spectroscopy and site directed mutagenesis experiments. Utilizing NMR titration data, we generated the structural models of S100B-FGF2 complex from the computational docking program, HADDOCK which were further proved stable during 15ns unrestrained molecular dynamics (MD) simulations. Isothermal titration calorimetry studies indicated S100B interaction with FGF2 is an entropically favored process implying dominant role of hydrophobic contacts at the protein-protein interface. Residue level information of S100B interaction with FGF2 was useful to understand the varied target recognition ability of S100B and further explained its role in effecting extracellular signaling diversity. Mechanistic insights into the S100B-FGF2 complex interface and cell-based assay studies involving mutants led us to conclude the novel role of S100B in FGF2 mediated FGFR1 receptor inactivation.
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==About this Structure==
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Structural insights into the interaction of human S100B and basic fibroblast growth factor (FGF2): Effects on FGFR1 receptor signaling.,Gupta AA, Chou RH, Li H, Yang LW, Yu C Biochim Biophys Acta. 2013 Dec;1834(12):2606-19. doi:, 10.1016/j.bbapap.2013.09.012. Epub 2013 Sep 22. PMID:24063890<ref>PMID:24063890</ref>
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[[2m49]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M49 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024063890</ref><references group="xtra"/><references/>
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</div>
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[[Category: Gupta, A A.]]
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<div class="pdbe-citations 2m49" style="background-color:#fffaf0;"></div>
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[[Category: Yu, C.]]
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[[Category: Cytokine-metal binding protein complex]]
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==See Also==
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[[Category: Fgf2]]
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*[[Fibroblast growth factor 3D structures|Fibroblast growth factor 3D structures]]
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[[Category: S100b]]
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*[[S100 proteins 3D structures|S100 proteins 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Gupta AA]]
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[[Category: Yu C]]

Current revision

Structural Insights into Human S100B and Basic Fibroblast Growth Factor (FGF2) Interaction

PDB ID 2m49

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