Nitrate reductase

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:48, 23 July 2019) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_2jim| PDB=2jim | SIZE=400| SCENE= |right|CAPTION=Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo (green) and molybdepterin guanine nucleotide (stick model), [[2jim]] }}
+
<StructureSection load='' size='350' side='right' caption='Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3- and molybdepterin guanine nucleotide (PDB entry [[2jiq]])' scene='49/490062/Cv/3'>
-
<!--
+
== Function ==
-
Please use the "3D" button above this box to insert a Jmol applet (molecule) on this page.
+
'''Nitrate reductase''' (NR) catalyzes the reduction of NO<sub>3</sub> to NO<sub>2</sub> using NADPH<ref>PMID:13152110</ref>. NR active site contains Mo atom. Four types of NR are known:<br />
-
Or use the four-green-boxes-button to insert scrollable text adjacent
+
'''eukaryotic assimilatory NR'''<br />
-
to a Jmol applet. Check out the other buttons as well!
+
'''bacterial cytoplasmic assimilatory''' (NAC)<br />
-
-->
+
'''bacterial membrane-bound respiratory''' (NAR)<br />
-
'''Nitrate reductase''' (NR) catalyzes the reduction of NO3 to NO2 using NADPH . NR active site contains Mo atom. Four types of NR are known: one eukaryotic assimilatory NR and 3 bacterial NRs – cytoplasmic assimilatory (NAC), membrane-bound respiratory (NAR) and periplasmic dissimilatory (NAP). The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR.
+
'''bacterial periplasmic dissimilatory''' (NAP).<br />
 +
The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR.
 +
 
 +
== Structural highlights ==
 +
<scene name='49/490062/Cv/7'>Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3 and 2 molybdepterin guanine nucleotides</scene>. NAP active site includes a <scene name='49/490062/Cv/8'>six-coordinated Mo atom which coordinates to 2 MGD molecules and Cys140</scene>. The <scene name='49/490062/Cv/9'>NO3 molecule is guided to the active site by a funnel leading to the Mo atom</scene><ref>PMID:18327621</ref>.
 +
 
 +
</StructureSection>
==3D structures of nitrate reductase==
==3D structures of nitrate reductase==
-
'''Bacterial periplasmic dissimilatory NR'''
+
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
 +
{{#tree:id=OrganizedByTopic|openlevels=0|
 +
 
 +
*Bacterial periplasmic dissimilatory NR
 +
 
 +
**[[2nap]], [[2jim]], [[2jio]], [[2jip]], [[2v3v]], [[2v45]] – DdNAP + MGD + Mo - ''Desulfovibrio desulfuricans''<br />
 +
**[[2jiq]] - DdNAP + NO3 + MGD + Mo<br />
 +
**[[2jir]] - DdNAP + CN + MGD + Mo<br />
 +
**[[1jni]] – NAP small subunit + heme – ''Haemophilus influenzae''<br />
 +
**[[1ogy]] - NAP + heme + MGD + Mo– ''Rhodobacter sphaeroides''<br />
 +
**[[2nya]] - EcNAP + MGD + Mo – ''Escherichia coli''<br />
 +
**[[3ml1]], [[3o5a]] - NAP catalytic subunit + NAPB + heme + MGD + Mo – ''Ralstonia eutropha''<br />
 +
**[[5yly]] – NR cytochrome b5 reductase domain + FAD – ''Ulva prolifera''<br />
 +
 
 +
*Bacterial respiratory NR
-
[[2nap]], [[2jim]], [[2jio]], [[2jip]], [[2v3v]], [[2v45]] – DdNAP + MGD + Mo - ''Desulfovibrio desulfuricans''<br />
+
**[[1q16]], [[1y5l]] – EcNAR α+β+γ chains + heme + MGD + Mo<br />
-
[[2jiq]] - DdNAP + NO3 + MGD + Mo<br />
+
**[[1r27]] - EcNAR α+β chains + MGD + Mo<br />
-
[[2jir]] - DdNAP + CN + MGD + Mo<br />
+
**[[1siw]] – EcNAR α+β+γ chains + heme<br />
-
[[1jni]] – NAP small subunit + heme – ''Haemophilus influenzae''<br />
+
**[[1y4z]], [[3egw]] - EcNAR α+β (mutant) + γ chains + inhibitor + heme + MGD + Mo<br />
-
[[1ogy]] - NAP + heme + MGD + Mo– ''Rhodobacter sphaeroides''<br />
+
**[[1y5i]], [[1y5n]] - EcNAR α+β + γ (mutant) chains + inhibitor + heme + MGD + Mo<br />
-
[[2nya]] - EcNAP + MGD + Mo – ''Escherichia coli''<br />
+
**[[3ir5]], [[3ir7]] - EcNAR α (mutant) +β + γ chains + inhibitor + heme + MGD + Mo<br />
-
[[3ml1]], [[3o5a]] - NAP catalytic subunit + NAPB + heme + MGD + Mo – ''Ralstonia eutropha''
+
**[[3ir6]] - EcNAR α (mutant) +β + γ chains + inhibitor + heme + GDP
-
'''Bacterial respiratory NR'''
+
*Eukaryotic assimilatory NR
-
[[1q16]] – EcNAR α+β+γ chains + heme + MGD + Mo<br />
+
**[[2bih]], [[2bii]] – NR MoCo-binding domain + MPT – ''Pichia angusta''
-
[[1r27]] - EcNAR α+β chains + MGD + Mo<br />
+
-
[[1siw]] – EcNAR α+β+γ chains + heme<br />
+
-
[[1y4z]], [[3egw]] - EcNAR α+β (mutant) + γ chains + inhibitor + heme + MGD + Mo<br />
+
-
[[1y5i]], [[1y5n]] - EcNAR α+β + γ (mutant) chains + inhibitor + heme + MGD + Mo<br />
+
-
[[3ir5]], [[3ir7]] - EcNAR α (mutant) +β + γ chains + inhibitor + heme + MGD + Mo<br />
+
-
[[3ir6]] - EcNAR α (mutant) +β + γ chains + inhibitor + heme + GDP
+
-
'''Eukaryotic assimilatory NR'''
+
*Eukaryotic NR
-
[[2bih]], [[2bii]] – NR MoCo-binding domain + MPT – ''Pichia angusta''
+
**[[1cne]] – cNR + FAD – corn<br />
 +
**[[1cnf]], [[2cnd]] – cNR (mutant) + FAD + ADP<br />
 +
}}
 +
== References ==
 +
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3- and molybdepterin guanine nucleotide (PDB entry 2jiq)

Drag the structure with the mouse to rotate

3D structures of nitrate reductase

Updated on 23-July-2019

References

  1. ↑ NICHOLAS DJ, NASON A. Molybdenum and nitrate reductase. II. Molybdenum as a constituent of nitrate reductase. J Biol Chem. 1954 Mar;207(1):353-60. PMID:13152110
  2. ↑ Najmudin S, Gonzalez PJ, Trincao J, Coelho C, Mukhopadhyay A, Cerqueira NM, Romao CC, Moura I, Moura JJ, Brondino CD, Romao MJ. Periplasmic nitrate reductase revisited: a sulfur atom completes the sixth coordination of the catalytic molybdenum. J Biol Inorg Chem. 2008 Mar 8;. PMID:18327621 doi:10.1007/s00775-008-0359-6

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

Retrieved from "http://52.214.119.220/wiki/index.php/Nitrate_reductase"
Personal tools