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| - | [[Image:2v46.gif|left|200px]]<br /><applet load="2v46" size="350" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[4v5a]] This PDB entry is obsolete and replaced by 4v5a |
| - | caption="2v46, resolution 3.50Å" />
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| - | '''STRUCTURE OF THE RIBOSOME RECYCLING FACTOR BOUND TO THE THERMUS THERMOPHILUS 70S RIBOSOME WITH MRNA, ASL-PHE AND TRNA-FMET (PART 1 OF 4). THIS FILE CONTAINS THE 30S SUBUNIT, MRNA, P-SITE ASL, E-SITE TRNA AND RRF FOR MOLECULE 1.'''<br />
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| - | ==Overview==
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| - | In bacteria, disassembly of the ribosome at the end of translation is facilitated by an essential protein factor termed ribosome recycling factor (RRF), which works in concert with elongation factor G. Here we describe the crystal structure of the Thermus thermophilus RRF bound to a 70S ribosomal complex containing a stop codon in the A site, a transfer RNA anticodon stem-loop in the P site and tRNA(fMet) in the E site. The work demonstrates that structures of translation factors bound to 70S ribosomes can be determined at reasonably high resolution. Contrary to earlier reports, we did not observe any RRF-induced changes in bridges connecting the two subunits. This suggests that such changes are not a direct requirement for or consequence of RRF binding but possibly arise from the subsequent stabilization of a hybrid state of the ribosome.
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| - | ==About this Structure==
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| - | 2V46 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V46 OCA].
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| - | ==Reference==
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| - | Crystal structure of the ribosome recycling factor bound to the ribosome., Weixlbaumer A, Petry S, Dunham CM, Selmer M, Kelley AC, Ramakrishnan V, Nat Struct Mol Biol. 2007 Aug;14(8):733-7. Epub 2007 Jul 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17660830 17660830]
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| - | [[Category: Protein complex]]
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| - | [[Category: Thermus thermophilus]]
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| - | [[Category: Dunham, C M.]]
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| - | [[Category: Kelley, A C.]]
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| - | [[Category: Petry, S.]]
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| - | [[Category: Ramakrishnan, V.]]
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| - | [[Category: Selmer, M.]]
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| - | [[Category: Weixlbaumer, A.]]
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| - | [[Category: MG]]
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| - | [[Category: ZN]]
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| - | [[Category: coiled coil]]
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| - | [[Category: metal-binding]]
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| - | [[Category: mrna]]
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| - | [[Category: protein biosynthesis]]
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| - | [[Category: recycling]]
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| - | [[Category: ribonucleoprotein]]
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| - | [[Category: ribosomal protein]]
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| - | [[Category: ribosome]]
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| - | [[Category: rna-binding]]
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| - | [[Category: rrf]]
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| - | [[Category: rrna-binding]]
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| - | [[Category: translation]]
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| - | [[Category: trna]]
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| - | [[Category: trna-binding]]
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| - | [[Category: zinc]]
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| - | [[Category: zinc-finger]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:53:11 2008''
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