4liw
From Proteopedia
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| - | {{STRUCTURE_4liw| PDB=4liw | SCENE= }} | ||
| - | ===CcmK1 Carboxysome Shell Protein from Synechocystis PCC6803, L11K Point Mutant=== | ||
| - | == | + | ==CcmK1 Carboxysome Shell Protein from Synechocystis PCC6803, L11K Point Mutant== |
| - | [[http://www.uniprot.org/uniprot/CCMK1_SYNY3 CCMK1_SYNY3 | + | <StructureSection load='4liw' size='340' side='right'caption='[[4liw]], [[Resolution|resolution]] 1.60Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4liw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803_substr._Kazusa Synechocystis sp. PCC 6803 substr. Kazusa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LIW FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4liw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4liw OCA], [https://pdbe.org/4liw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4liw RCSB], [https://www.ebi.ac.uk/pdbsum/4liw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4liw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CCMK1_SYNY3 CCMK1_SYNY3] May be involved in the formation of the carboxysome, a polyhedral inclusion where RuBisCO is sequestered (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The carboxysome is a giant protein complex that acts as a metabolic organelle in cyanobacteria and some chemoautotrophs. Its outer structure is formed by the assembly of thousands of copies of hexameric shell protein subunits into a molecular layer. The structure determination of a CcmK1 shell protein mutant (L11K) from the beta-carboxysome of the cyanobacterium Synechocystis PCC6803 led to challenges in structure determination. Twinning, noncrystallographic symmetry and packing of hexameric units in a special arrangement led to initial difficulties in space-group assignment. The correct space group was clarified after initial model refinement revealed additional symmetry. This study provides an instructive example in which broken symmetry requires a new choice of unit-cell origin in order to identify the highest symmetry space group. An additional observation related to the packing arrangement of molecules in this crystal suggests that these hexameric shell proteins might have lower internal symmetry than previously believed. | ||
| - | + | A challenging interpretation of a hexagonally layered protein structure.,Thompson MC, Yeates TO Acta Crystallogr D Biol Crystallogr. 2014 Jan;70(Pt 1):203-8. doi:, 10.1107/S139900471302422X. Epub 2013 Dec 24. PMID:24419393<ref>PMID:24419393</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 4liw" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | + | [[Category: Large Structures]] | |
| + | [[Category: Synechocystis sp. PCC 6803 substr. Kazusa]] | ||
| + | [[Category: Thompson MC]] | ||
| + | [[Category: Yeates TO]] | ||
Current revision
CcmK1 Carboxysome Shell Protein from Synechocystis PCC6803, L11K Point Mutant
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