3b0i

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{{STRUCTURE_3b0i| PDB=3b0i | SCENE= }}
 
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===Crystal structure of recombinant human alpha lactalbumin===
 
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{{ABSTRACT_PUBMED_23155056}}
 
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==Function==
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==Crystal structure of recombinant human alpha lactalbumin==
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[[http://www.uniprot.org/uniprot/LALBA_HUMAN LALBA_HUMAN]] Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.
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<StructureSection load='3b0i' size='340' side='right'caption='[[3b0i]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3b0i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B0I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B0I FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b0i OCA], [https://pdbe.org/3b0i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b0i RCSB], [https://www.ebi.ac.uk/pdbsum/3b0i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b0i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LALBA_HUMAN LALBA_HUMAN] Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Addition of an extra methionine at the N-terminus by recombinant expression of alpha-lactalbumin in Escherichia coli significantly destabilizes the protein, and this destabilization has hampered mutational analyses such as the mutational phi-value analysis of the protein. Deletion of residue 1 from the recombinant form recovers the stability in human and goat alpha-lactalbumin. Here, we thus determined the crystal structures of the residue 1-deletion variants of recombinant human and goat alpha-lactalbumin, and compared the structures with those of the authentic and recombinant forms. The results demonstrate the importance of the N-terminal backbone structure and hydrogen-bonding pattern for the stability of alpha-lactalbumin.
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==About this Structure==
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Structural insights into the stability perturbations induced by N-terminal variation in human and goat alpha-lactalbumin.,Makabe K, Nakamura T, Kuwajima K Protein Eng Des Sel. 2013 Feb;26(2):165-70. doi: 10.1093/protein/gzs093. Epub, 2012 Nov 14. PMID:23155056<ref>PMID:23155056</ref>
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[[3b0i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B0I OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023155056</ref><references group="xtra"/><references/>
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</div>
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[[Category: Human]]
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<div class="pdbe-citations 3b0i" style="background-color:#fffaf0;"></div>
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[[Category: Makabe, K.]]
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[[Category: Calcium binding protein]]
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==See Also==
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[[Category: Glycoprotein]]
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*[[Alpha-lactalbumin 3D structures|Alpha-lactalbumin 3D structures]]
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[[Category: Metal binding protein]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Makabe K]]

Current revision

Crystal structure of recombinant human alpha lactalbumin

PDB ID 3b0i

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