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2z2s

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[[Image:2z2s.jpg|left|200px]]<br /><applet load="2z2s" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2z2s, resolution 2.7&Aring;" />
 
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'''Crystal Structure of Rhodobacter sphaeroides SigE in complex with the anti-sigma ChrR'''<br />
 
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==Overview==
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==Crystal Structure of Rhodobacter sphaeroides SigE in complex with the anti-sigma ChrR==
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<StructureSection load='2z2s' size='340' side='right'caption='[[2z2s]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2z2s]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhos4 Rhos4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z2S FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2q1z|2q1z]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpoE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272943 RHOS4]), chrR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272943 RHOS4])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z2s OCA], [https://pdbe.org/2z2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z2s RCSB], [https://www.ebi.ac.uk/pdbsum/2z2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z2s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/RPOE_RHOS4 RPOE_RHOS4]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by a cognate anti-sigma factor until released. Sigma-E controls a transcriptional response to singlet oxygen, a by-product of photosynthesis; its continuous activity requires constant exposure to singlet oxygen. The regulon has about 180 genes that protect against or repair damage induced by singlet oxygen, including itself and rpoH2, a heat shock-responsive sigma factor.<ref>PMID:11676534</ref> <ref>PMID:15855269</ref> <ref>PMID:17803943</ref> [[https://www.uniprot.org/uniprot/CHRR_RHOS4 CHRR_RHOS4]] Anti-sigma factor that inhibits the activity of the extracytoplasmic function (ECF) sigma-E factor (RpoE), thereby indirectly regulating the transcription of the cycA and rpoE genes. ECF sigma factors are held in an inactive form by a cognate anti-sigma factor.<ref>PMID:11676534</ref> <ref>PMID:15855269</ref> <ref>PMID:17803943</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z2/2z2s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z2s ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
A transcriptional response to singlet oxygen in Rhodobacter sphaeroides is controlled by the group IV sigma factor sigma(E) and its cognate anti-sigma ChrR. Crystal structures of the sigma(E)/ChrR complex reveal a modular, two-domain architecture for ChrR. The ChrR N-terminal anti-sigma domain (ASD) binds a Zn(2+) ion, contacts sigma(E), and is sufficient to inhibit sigma(E)-dependent transcription. The ChrR C-terminal domain adopts a cupin fold, can coordinate an additional Zn(2+), and is required for the transcriptional response to singlet oxygen. Structure-based sequence analyses predict that the ASD defines a common structural fold among predicted group IV anti-sigmas. These ASDs are fused to diverse C-terminal domains that are likely involved in responding to specific environmental signals that control the activity of their cognate sigma factor.
A transcriptional response to singlet oxygen in Rhodobacter sphaeroides is controlled by the group IV sigma factor sigma(E) and its cognate anti-sigma ChrR. Crystal structures of the sigma(E)/ChrR complex reveal a modular, two-domain architecture for ChrR. The ChrR N-terminal anti-sigma domain (ASD) binds a Zn(2+) ion, contacts sigma(E), and is sufficient to inhibit sigma(E)-dependent transcription. The ChrR C-terminal domain adopts a cupin fold, can coordinate an additional Zn(2+), and is required for the transcriptional response to singlet oxygen. Structure-based sequence analyses predict that the ASD defines a common structural fold among predicted group IV anti-sigmas. These ASDs are fused to diverse C-terminal domains that are likely involved in responding to specific environmental signals that control the activity of their cognate sigma factor.
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==About this Structure==
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A conserved structural module regulates transcriptional responses to diverse stress signals in bacteria.,Campbell EA, Greenwell R, Anthony JR, Wang S, Lim L, Das K, Sofia HJ, Donohue TJ, Darst SA Mol Cell. 2007 Sep 7;27(5):793-805. PMID:17803943<ref>PMID:17803943</ref>
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2Z2S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Zn+Binding+Site+For+Residue+B+1'>AC1</scene>, <scene name='pdbsite=AC2:Zn+Binding+Site+For+Residue+H+1'>AC2</scene>, <scene name='pdbsite=AC3:Zn+Binding+Site+For+Residue+D+1'>AC3</scene>, <scene name='pdbsite=AC4:Zn+Binding+Site+For+Residue+F+1'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+E+1'>AC5</scene> and <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+G+2'>AC6</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z2S OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A conserved structural module regulates transcriptional responses to diverse stress signals in bacteria., Campbell EA, Greenwell R, Anthony JR, Wang S, Lim L, Das K, Sofia HJ, Donohue TJ, Darst SA, Mol Cell. 2007 Sep 7;27(5):793-805. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17803943 17803943]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 2z2s" style="background-color:#fffaf0;"></div>
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[[Category: Rhodobacter sphaeroides]]
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== References ==
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[[Category: Campbell, E A.]]
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<references/>
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[[Category: Darst, S A.]]
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__TOC__
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[[Category: SO4]]
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</StructureSection>
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[[Category: ZN]]
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[[Category: Large Structures]]
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[[Category: activator]]
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[[Category: Rhos4]]
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[[Category: anti-sigma factor]]
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[[Category: Campbell, E A]]
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[[Category: cupin fold]]
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[[Category: Darst, S A]]
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[[Category: dna-binding]]
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[[Category: Activator]]
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[[Category: ecf sigma factor]]
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[[Category: Anti-sigma factor]]
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[[Category: metal-binding]]
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[[Category: Cupin fold]]
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[[Category: transcription]]
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[[Category: Dna-binding]]
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[[Category: transcription regulation]]
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[[Category: Ecf sigma factor]]
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[[Category: zinc]]
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[[Category: Metal-binding]]
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[[Category: zinc binding transcription factor]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:59:48 2008''
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[[Category: Zinc binding transcription factor]]

Current revision

Crystal Structure of Rhodobacter sphaeroides SigE in complex with the anti-sigma ChrR

PDB ID 2z2s

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