4njc
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==RNA Polymerase interacting protein YkzG from Geobacillus stearothermophilus== | |
| + | <StructureSection load='4njc' size='340' side='right'caption='[[4njc]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4njc]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NJC FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4njc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4njc OCA], [https://pdbe.org/4njc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4njc RCSB], [https://www.ebi.ac.uk/pdbsum/4njc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4njc ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RPOY_GEOS3 RPOY_GEOS3] A non-essential component of RNA polymerase (RNAP) (Probable). Has a similar structure to bacteriophage T7 protein Gp2 (AC P03704), which is known to bind to RNAP in the DNA binding-cleft. Unlike Gp2 however, this protein does not inhibit transcription inititation (PubMed:25092033).<ref>PMID:25092033</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | RNA polymerase in bacteria is a multisubunit protein complex that is essential for gene expression. We have identified a new subunit of RNA polymerase present in the high A+T firmicutes phylum of Gram positive bacteria, and have named it . Previously had been identified as a small protein (omega1) that co-purified with RNA polymerase. We have solved the structure of by X-ray crystallography and show that it is not an omega subunit. Rather, bears remarkable similarity to the Gp2 family of phage proteins involved in the inhibition of host cell transcription following infection. Deletion of shows no phenotype and has no effect on the transcriptional profile of the cell. Determination of the location of within the assembly of RNA polymerase core by single particle analysis suggests it binds towards the downstream side of the DNA binding cleft. Due to the structural similarity of with Gp2, and the fact they bind similar regions of RNA polymerase, we hypothesise that may serve a role in protection from phage infection. | ||
| - | + | : A new subunit of RNA polymerase found in Gram positive bacteria.,Keller A, Yang X, Wiedermannova J, Delumeau O, Krasny L, Lewis PJ J Bacteriol. 2014 Aug 4. pii: JB.02020-14. PMID:25092033<ref>PMID:25092033</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4njc" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Geobacillus stearothermophilus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Keller AN]] | ||
| + | [[Category: Lewis PJ]] | ||
Current revision
RNA Polymerase interacting protein YkzG from Geobacillus stearothermophilus
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