2z8z
From Proteopedia
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| - | [[Image:2z8z.gif|left|200px]]<br /><applet load="2z8z" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2z8z, resolution 1.80Å" /> | ||
| - | '''Crystal structure of a platinum-bound S445C mutant of Pseudomonas sp. MIS38 lipase'''<br /> | ||
| - | == | + | ==Crystal structure of a platinum-bound S445C mutant of Pseudomonas sp. MIS38 lipase== |
| + | <StructureSection load='2z8z' size='340' side='right'caption='[[2z8z]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2z8z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._MIS38 Pseudomonas sp. MIS38]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z8Z FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8z OCA], [https://pdbe.org/2z8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z8z RCSB], [https://www.ebi.ac.uk/pdbsum/2z8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z8z ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9RBY1_9PSED Q9RBY1_9PSED] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z8/2z8z_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z8z ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism. | The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism. | ||
| - | + | Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation.,Angkawidjaja C, You DJ, Matsumura H, Kuwahara K, Koga Y, Takano K, Kanaya S FEBS Lett. 2007 Oct 30;581(26):5060-4. Epub 2007 Oct 1. PMID:17923123<ref>PMID:17923123</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2z8z" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Lipase 3D Structures|Lipase 3D Structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas sp. MIS38]] | ||
| + | [[Category: Angkawidjaja C]] | ||
| + | [[Category: Kanaya S]] | ||
| + | [[Category: Koga Y]] | ||
| + | [[Category: Matsumura H]] | ||
| + | [[Category: Takano K]] | ||
| + | [[Category: You DJ]] | ||
Current revision
Crystal structure of a platinum-bound S445C mutant of Pseudomonas sp. MIS38 lipase
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