4n49

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{{STRUCTURE_4n49| PDB=4n49 | SCENE= }}
 
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===Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 Protein in complex with m7GpppG and SAM===
 
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{{ABSTRACT_PUBMED_24402442}}
 
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==About this Structure==
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==Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 Protein in complex with m7GpppG and SAM==
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[[4n49]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N49 OCA].
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<StructureSection load='4n49' size='340' side='right'caption='[[4n49]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4n49]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N49 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N49 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MGT:7N-METHYL-8-HYDROGUANOSINE-5-TRIPHOSPHATE'>MGT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n49 OCA], [https://pdbe.org/4n49 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n49 RCSB], [https://www.ebi.ac.uk/pdbsum/4n49 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n49 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CMTR1_HUMAN CMTR1_HUMAN] S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m(7)GpppG-capped mRNA and small nuclear RNA (snRNA) to produce m(7)GpppRm (cap1). Displays a preference for cap0 transcripts. Cap1 modification is linked to higher levels of translation. May be involved in the interferon response pathway.<ref>PMID:18533109</ref> <ref>PMID:20713356</ref> <ref>PMID:21310715</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 5' cap of human messenger RNA contains 2'-O-methylation of the first and often second transcribed nucleotide that is important for its processing, translation and stability. Human enzymes that methylate these nucleotides, termed CMTr1 and CMTr2, respectively, have recently been identified. However, the structures of these enzymes and their mechanisms of action remain unknown. In the present study, we solve the crystal structures of the active CMTr1 catalytic domain in complex with a methyl group donor and a capped oligoribonucleotide, thereby revealing the mechanism of specific recognition of capped RNA. This mechanism differs significantly from viral enzymes, thus providing a framework for their specific targeting. Based on the crystal structure of CMTr1, a comparative model of the CMTr2 catalytic domain is generated. This model, together with mutational analysis, leads to the identification of residues involved in RNA and methyl group donor binding.
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==Reference==
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Structural analysis of human 2'-O-ribose methyltransferases involved in mRNA cap structure formation.,Smietanski M, Werner M, Purta E, Kaminska KH, Stepinski J, Darzynkiewicz E, Nowotny M, Bujnicki JM Nat Commun. 2014 Jan 9;5:3004. doi: 10.1038/ncomms4004. PMID:24402442<ref>PMID:24402442</ref>
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<ref group="xtra">PMID:024402442</ref><references group="xtra"/><references/>
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[[Category: Bujnicki, J M.]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Darzynkiewicz, E.]]
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</div>
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[[Category: Kaminska, K H.]]
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<div class="pdbe-citations 4n49" style="background-color:#fffaf0;"></div>
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[[Category: Nowotny, M.]]
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== References ==
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[[Category: Purta, E.]]
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<references/>
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[[Category: Smietanski, M.]]
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__TOC__
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[[Category: Stepinski, J.]]
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</StructureSection>
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[[Category: Werener, M.]]
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[[Category: Homo sapiens]]
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[[Category: Methyltransferase]]
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[[Category: Large Structures]]
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[[Category: Mrna]]
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[[Category: Bujnicki JM]]
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[[Category: Mrna cap methylation]]
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[[Category: Darzynkiewicz E]]
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[[Category: Transferase]]
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[[Category: Kaminska KH]]
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[[Category: Nowotny M]]
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[[Category: Purta E]]
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[[Category: Smietanski M]]
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[[Category: Stepinski J]]
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[[Category: Werener M]]

Current revision

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 Protein in complex with m7GpppG and SAM

PDB ID 4n49

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