3zif

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{{STRUCTURE_3zif| PDB=3zif | SCENE= }}
 
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===Cryo-EM structures of two intermediates provide insight into adenovirus assembly and disassembly===
 
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==Function==
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==Cryo-EM structures of two intermediates provide insight into adenovirus assembly and disassembly==
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[[http://www.uniprot.org/uniprot/CAPSH_ADEB3 CAPSH_ADEB3]] Major capsid protein that self-associates to form 240 hexon trimers, each in the shape of a hexagon, building most of the pseudo T=25 capsid. Assembled into trimeric units with the help of the chaperone shutoff protein. Transported by pre-protein VI to the nucleus where it associates with other structural proteins to form an empty capsid. Might be involved, through its interaction with host dyneins, in the intracellular microtubule-dependent transport of incoming viral capsid to the nucleus (By similarity).
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<SX load='3zif' size='340' side='right' viewer='molstar' caption='[[3zif]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3zif]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovine_adenovirus_3 Bovine adenovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZIF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zif OCA], [https://pdbe.org/3zif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zif RCSB], [https://www.ebi.ac.uk/pdbsum/3zif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zif ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAPSH_ADEB3 CAPSH_ADEB3] Major capsid protein that self-associates to form 240 hexon trimers, each in the shape of a hexagon, building most of the pseudo T=25 capsid. Assembled into trimeric units with the help of the chaperone shutoff protein. Transported by pre-protein VI to the nucleus where it associates with other structural proteins to form an empty capsid. Might be involved, through its interaction with host dyneins, in the intracellular microtubule-dependent transport of incoming viral capsid to the nucleus (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are localized in the fivefold vertex region, while their facet structures are identical. The overall facet structure of BAd3 exhibits a similar structure to human Ads; however, BAd3 protein IX has a unique conformation. Mass spectrometry and cryo-electron tomography analyses indicate that one intermediate structure represents the stage during DNA encapsidation, whilst the other intermediate structure represents a later stage. These results also suggest that cleavage of precursor protein VI occurs during, rather than after, the DNA encapsidation process. Overall, our results provide insights into the mechanism of Ad assembly, and allow the first structural comparison between human and nonhuman Ads at backbone level.
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==About this Structure==
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Cryo-EM structures of two bovine adenovirus type 3 intermediates.,Cheng L, Huang X, Li X, Xiong W, Sun W, Yang C, Zhang K, Wang Y, Liu H, Huang X, Ji G, Sun F, Zheng C, Zhu P Virology. 2014 Feb;450-451:174-81. doi: 10.1016/j.virol.2013.12.012. Epub 2014, Jan 3. PMID:24503080<ref>PMID:24503080</ref>
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[[3zif]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovine_adenovirus_3 Bovine adenovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIF OCA].
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3zif" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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== References ==
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<references/>
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__TOC__
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</SX>
[[Category: Bovine adenovirus 3]]
[[Category: Bovine adenovirus 3]]
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[[Category: Cheng, L.]]
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[[Category: Large Structures]]
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[[Category: Huang, X.]]
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[[Category: Cheng L]]
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[[Category: Ji, G.]]
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[[Category: Huang X]]
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[[Category: Li, X.]]
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[[Category: Ji G]]
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[[Category: Liu, H.]]
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[[Category: Li X]]
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[[Category: Sun, F.]]
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[[Category: Liu H]]
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[[Category: Sun, W.]]
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[[Category: Sun F]]
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[[Category: Wang, Y.]]
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[[Category: Sun W]]
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[[Category: Xiong, W.]]
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[[Category: Wang Y]]
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[[Category: Yang, C.]]
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[[Category: Xiong W]]
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[[Category: Zhang, K.]]
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[[Category: Yang C]]
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[[Category: Zheng, C.]]
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[[Category: Zhang K]]
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[[Category: Zhu, P.]]
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[[Category: Zheng C]]
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[[Category: Assembly intermediate]]
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[[Category: Zhu P]]
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[[Category: Virus]]
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Current revision

Cryo-EM structures of two intermediates provide insight into adenovirus assembly and disassembly

3zif, resolution 4.50Å

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OCA

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