329d

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[[Image:329d.gif|left|200px]]<br /><applet load="329d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="329d, resolution 2.700&Aring;" />
 
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'''EFFECT OF CYTOSINE METHYLATION ON DNA-DNA RECOGNITION AT CPG STEPS'''<br />
 
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==Overview==
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==EFFECT OF CYTOSINE METHYLATION ON DNA-DNA RECOGNITION AT CPG STEPS==
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Although DNA methylation is a fundamental mechanism for repressing genetic activity, the influence of methyl groups on DNA conformation is found to be small. In this study, the role of cytosine methylation is analysed in the context of DNA condensation by examining its influence on DNA-DNA recognition processes. Previously CpG sites were found to act as sequence determinants for the close and specific self-fit of B-DNA helices into cross-overs. In the present study, the crystal structure of the B-DNA dodecamer d(ACCGCCGGCGCC) methylated at its central CpG sequence shows that the methyl groups do not interfere with DNA self-fitting. In contrast, the two methyl groups form a clamp, which traps the incoming phosphate in the groove-backbone interaction. This geometry allows the formation of two new C-H...O hydrogen bonds between the methyl groups and the anionic oxygen atoms of the phosphate, which may further stabilize the interaction. This finding relates cytosine methylation to the formation of higher-order DNA structures and could provide new insights for understanding the mode of action of DNA methylation in genetic inactivation.
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<StructureSection load='329d' size='340' side='right'caption='[[329d]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[329d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=329D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=329D FirstGlance]. <br>
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329D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=329D OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=329d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=329d OCA], [https://pdbe.org/329d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=329d RCSB], [https://www.ebi.ac.uk/pdbsum/329d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=329d ProSAT]</span></td></tr>
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Effect of cytosine methylation on DNA-DNA recognition at CpG steps., Mayer-Jung C, Moras D, Timsit Y, J Mol Biol. 1997 Jul 18;270(3):328-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9237900 9237900]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Mayer-Jung, C.]]
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</StructureSection>
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[[Category: Moras, D.]]
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[[Category: Large Structures]]
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[[Category: Timsit, Y.]]
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[[Category: Mayer-Jung C]]
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[[Category: b-dna]]
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[[Category: Moras D]]
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[[Category: double helix]]
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[[Category: Timsit Y]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:02 2008''
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Current revision

EFFECT OF CYTOSINE METHYLATION ON DNA-DNA RECOGNITION AT CPG STEPS

PDB ID 329d

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