392d

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[[Image:392d.gif|left|200px]]<br /><applet load="392d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="392d, resolution 3.000&Aring;" />
 
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'''STRUCTURAL VARIABILITY AND NEW INTERMOLECULAR INTERACTIONS OF Z-DNA IN CRYSTALS OF D(PCPGPCPGPCPG)'''<br />
 
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==Overview==
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==STRUCTURAL VARIABILITY AND NEW INTERMOLECULAR INTERACTIONS OF Z-DNA IN CRYSTALS OF D(PCPGPCPGPCPG)==
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We have determined the single crystal x-ray structure of the synthetic DNA hexamer d(pCpGpCpGpCpG) in two different crystal forms. The hexamer pCGCGCG has the Z-DNA conformation and in both cases the asymmetric unit contains more than one Z-DNA duplex. Crystals belong to the space group C222(1) with a = 69.73, b = 52.63, and c = 26.21 A, and to the space group P2(1) with a = 49.87, b = 41.26, c = 21.91 A, and gamma = 97.12 degrees. Both crystals show new crystal packing modes. The molecules also show striking new features when compared with previously determined Z-DNA structures: 1) the bases in one duplex have a large inclination with respect to the helical axis, which alters the overall shape of the molecule. 2) Some cytosine nitrogens interact by hydrogen bonding with phosphates in neighbor molecules. Similar base-phosphate interactions had been previously detected in some B-DNA crystals. 3) Basepair stacking between the ends of neighbor molecules is variable and no helical continuity is maintained between contiguous hexamer duplexes.
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<StructureSection load='392d' size='340' side='right'caption='[[392d]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[392d]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=392D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=392D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=392d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=392d OCA], [https://pdbe.org/392d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=392d RCSB], [https://www.ebi.ac.uk/pdbsum/392d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=392d ProSAT]</span></td></tr>
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</table>
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==About this Structure==
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==See Also==
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392D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=392D OCA].
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*[[Z-DNA|Z-DNA]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural variability and new intermolecular interactions of Z-DNA in crystals of d(pCpGpCpGpCpG)., Malinina L, Tereshko V, Ivanova E, Subirana JA, Zarytova V, Nekrasov Y, Biophys J. 1998 May;74(5):2482-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9591674 9591674]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Ivanova E]]
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[[Category: Ivanova, E.]]
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[[Category: Malinina L]]
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[[Category: Malinina, L.]]
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[[Category: Nekrasov Y]]
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[[Category: Nekrasov, Y.]]
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[[Category: Subirana JA]]
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[[Category: Subirana, J A.]]
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[[Category: Tereshko V]]
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[[Category: Tereshko, V.]]
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[[Category: Zarytova V]]
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[[Category: Zarytova, V.]]
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[[Category: MG]]
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[[Category: double helix]]
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[[Category: z-dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:34 2008''
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Current revision

STRUCTURAL VARIABILITY AND NEW INTERMOLECULAR INTERACTIONS OF Z-DNA IN CRYSTALS OF D(PCPGPCPGPCPG)

PDB ID 392d

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