397d

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[[Image:397d.jpg|left|200px]]<br /><applet load="397d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="397d, resolution 1.300&Aring;" />
 
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'''A 1.3 A RESOLUTION CRYSTAL STRUCTURE OF THE HIV-1 TRANS-ACTIVATION RESPONSE REGION RNA STEM REVEALS A METAL ION-DEPENDENT BULGE CONFORMATION'''<br />
 
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==Overview==
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==A 1.3 A RESOLUTION CRYSTAL STRUCTURE OF THE HIV-1 TRANS-ACTIVATION RESPONSE REGION RNA STEM REVEALS A METAL ION-DEPENDENT BULGE CONFORMATION==
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The crystal structure of an HIV-1 trans-activation response region (TAR) RNA fragment containing the binding site for the trans-activation protein Tat has been determined to 1.3-A resolution. In this crystal structure, the characteristic UCU bulge of TAR adopts a conformation that is stabilized by three divalent calcium ions and differs from those determined previously by solution NMR. One metal ion, crucial to the loop conformation, binds directly to three phosphates in the loop region. The structure emphasizes the influence of metal ion binding on RNA structure and, given the abundance of divalent metal ion in the cell, raises the question of whether metal ions play a role in the conformation of TAR RNA and the interaction of TAR with Tat and cyclin T in vivo.
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<StructureSection load='397d' size='340' side='right'caption='[[397d]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[397d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=397D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=397D FirstGlance]. <br>
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397D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=397D OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=397d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=397d OCA], [https://pdbe.org/397d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=397d RCSB], [https://www.ebi.ac.uk/pdbsum/397d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=397d ProSAT]</span></td></tr>
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A 1.3-A resolution crystal structure of the HIV-1 trans-activation response region RNA stem reveals a metal ion-dependent bulge conformation., Ippolito JA, Steitz TA, Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9819-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9707559 9707559]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Ippolito, J A.]]
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</StructureSection>
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[[Category: Steitz, T A.]]
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[[Category: Large Structures]]
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[[Category: CA]]
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[[Category: Ippolito JA]]
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[[Category: double helix]]
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[[Category: Steitz TA]]
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[[Category: overhanging bases]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:37 2008''
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Current revision

A 1.3 A RESOLUTION CRYSTAL STRUCTURE OF THE HIV-1 TRANS-ACTIVATION RESPONSE REGION RNA STEM REVEALS A METAL ION-DEPENDENT BULGE CONFORMATION

PDB ID 397d

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