3b5d

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[[Image:3b5d.jpg|left|200px]]<br /><applet load="3b5d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="3b5d, resolution 3.8&Aring;" />
 
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'''EmrE multidrug transporter in complex with TPP, C2 crystal form'''<br />
 
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==Overview==
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==EmrE multidrug transporter in complex with TPP, C2 crystal form==
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EmrE, a multidrug transporter from Escherichia coli, functions as a homodimer of a small four-transmembrane protein. The membrane insertion topology of the two monomers is controversial. Although the EmrE protein was reported to have a unique orientation in the membrane, models based on electron microscopy and now defunct x-ray structures, as well as recent biochemical studies, posit an antiparallel dimer. We have now reanalyzed our x-ray data on EmrE. The corrected structures in complex with a transport substrate are highly similar to the electron microscopy structure. The first three transmembrane helices from each monomer surround the substrate binding chamber, whereas the fourth helices participate only in dimer formation. Selenomethionine markers clearly indicate an antiparallel orientation for the monomers, supporting a "dual topology" model.
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<StructureSection load='3b5d' size='340' side='right'caption='[[3b5d]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[3b5d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B5D FirstGlance]. <br>
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3B5D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=P4P:'>P4P</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5D OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b5d OCA], [https://pdbe.org/3b5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b5d RCSB], [https://www.ebi.ac.uk/pdbsum/3b5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b5d ProSAT]</span></td></tr>
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X-ray structure of EmrE supports dual topology model., Chen YJ, Pornillos O, Lieu S, Ma C, Chen AP, Chang G, Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):18999-9004. Epub 2007 Nov, 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18024586 18024586]
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</table>
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[[Category: Escherichia coli]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>
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[[Category: Chang, G.]]
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== Evolutionary Conservation ==
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[[Category: Chen, Y J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: P4P]]
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Check<jmol>
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[[Category: antiport]]
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<jmolCheckbox>
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[[Category: helical membrane protein]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/3b5d_consurf.spt"</scriptWhenChecked>
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[[Category: inner membrane]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: joint center for innovative membrane protein technologies]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: multidrug resistance transporter]]
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</jmolCheckbox>
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[[Category: smr]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b5d ConSurf].
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[[Category: transmembrane]]
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<div style="clear:both"></div>
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== References ==
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:03:24 2008''
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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[[Category: Chang G]]
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[[Category: Chen YJ]]

Current revision

EmrE multidrug transporter in complex with TPP, C2 crystal form

PDB ID 3b5d

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