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3b7n

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(New page: 200px<br /><applet load="3b7n" size="350" color="white" frame="true" align="right" spinBox="true" caption="3b7n, resolution 1.860&Aring;" /> '''Crystal Structure o...)
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[[Image:3b7n.jpg|left|200px]]<br /><applet load="3b7n" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="3b7n, resolution 1.860&Aring;" />
 
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'''Crystal Structure of Yeast Sec14 Homolog Sfh1 in Complex with Phosphatidylinositol'''<br />
 
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==Overview==
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==Crystal Structure of Yeast Sec14 Homolog Sfh1 in Complex with Phosphatidylinositol==
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Sec14, the major yeast phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer protein, regulates essential interfaces between lipid metabolism and membrane trafficking from the trans-Golgi network (TGN). How Sec14 does so remains unclear. We report that Sec14 binds PtdIns and PtdCho at distinct (but overlapping) sites, and both PtdIns- and PtdCho-binding activities are essential Sec14 activities. We further show both activities must reside within the same molecule to reconstitute a functional Sec14 and for effective Sec14-mediated regulation of phosphoinositide homeostasis in vivo. This regulation is uncoupled from PtdIns-transfer activity and argues for an interfacial presentation mode for Sec14-mediated potentiation of PtdIns kinases. Such a regulatory role for Sec14 is a primary counter to action of the Kes1 sterol-binding protein that antagonizes PtdIns 4-OH kinase activity in vivo. Collectively, these findings outline functional mechanisms for the Sec14 superfamily and reveal additional layers of complexity for regulating phosphoinositide homeostasis in eukaryotes.
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<StructureSection load='3b7n' size='340' side='right'caption='[[3b7n]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[3b7n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B7N FirstGlance]. <br>
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3B7N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=B7N:'>B7N</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Po4+Binding+Site+For+Residue+A+311'>AC1</scene>, <scene name='pdbsite=AC2:Po4+Binding+Site+For+Residue+A+312'>AC2</scene>, <scene name='pdbsite=AC3:Act+Binding+Site+For+Residue+A+313'>AC3</scene> and <scene name='pdbsite=AC4:B7n+Binding+Site+For+Residue+A+314'>AC4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B7N OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=B7N:(1R)-2-{[(S)-HYDROXY{[(1S,2R,3R,4S,5S,6R)-2,3,4,5,6-PENTAHYDROXYCYCLOHEXYL]OXY}PHOSPHORYL]OXY}-1-[(OCTADECANOYLOXY)METHYL]ETHYL+(9Z)-OCTADEC-9-ENOATE'>B7N</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b7n OCA], [https://pdbe.org/3b7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b7n RCSB], [https://www.ebi.ac.uk/pdbsum/3b7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b7n ProSAT]</span></td></tr>
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Functional anatomy of phospholipid binding and regulation of phosphoinositide homeostasis by proteins of the sec14 superfamily., Schaaf G, Ortlund EA, Tyeryar KR, Mousley CJ, Ile KE, Garrett TA, Ren J, Woolls MJ, Raetz CR, Redinbo MR, Bankaitis VA, Mol Cell. 2008 Feb 1;29(2):191-206. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18243114 18243114]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/YKJ1_YEAST YKJ1_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b7/3b7n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b7n ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Bankaitis V]]
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[[Category: Bankaitis, V.]]
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[[Category: Ortlund EA]]
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[[Category: Ortlund, E A.]]
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[[Category: Redinbo MR]]
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[[Category: Redinbo, M R.]]
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[[Category: Schaaf G]]
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[[Category: Schaaf, G.]]
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[[Category: ACT]]
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[[Category: B7N]]
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[[Category: PO4]]
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[[Category: golgi]]
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[[Category: phosphatidylinositol]]
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[[Category: phospholipid]]
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[[Category: sec14]]
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[[Category: signaling protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:04:00 2008''
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Current revision

Crystal Structure of Yeast Sec14 Homolog Sfh1 in Complex with Phosphatidylinositol

PDB ID 3b7n

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