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3b8n

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[[Image:3b8n.jpg|left|200px]]<br /><applet load="3b8n" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="3b8n, resolution 3.100&Aring;" />
 
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'''Structure of FepE- Bacterial Polysaccharide Co-polymerase'''<br />
 
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==Overview==
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==Structure of FepE- Bacterial Polysaccharide Co-polymerase==
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The chain length distribution of complex polysaccharides present on the bacterial surface is determined by polysaccharide co-polymerases (PCPs) anchored in the inner membrane. We report crystal structures of the periplasmic domains of three PCPs that impart substantially different chain length distributions to surface polysaccharides. Despite very low sequence similarities, they have a common protomer structure with a long central alpha-helix extending 100 A into the periplasm. The protomers self-assemble into bell-shaped oligomers of variable sizes, with a large internal cavity. Electron microscopy shows that one of the full-length PCPs has a similar organization as that observed in the crystal for its periplasmic domain alone. Functional studies suggest that the top of the PCP oligomers is an important region for determining polysaccharide modal length. These structures provide a detailed view of components of the bacterial polysaccharide assembly machinery.
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<StructureSection load='3b8n' size='340' side='right'caption='[[3b8n]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[3b8n]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B8N FirstGlance]. <br>
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3B8N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8N OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b8n OCA], [https://pdbe.org/3b8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b8n RCSB], [https://www.ebi.ac.uk/pdbsum/3b8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b8n ProSAT], [https://www.topsan.org/Proteins/BSGI/3b8n TOPSAN]</span></td></tr>
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==Reference==
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</table>
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Bacterial polysaccharide co-polymerases share a common framework for control of polymer length., Tocilj A, Munger C, Proteau A, Morona R, Purins L, Ajamian E, Wagner J, Papadopoulos M, Van Den Bosch L, Rubinstein JL, Fethiere J, Matte A, Cygler M, Nat Struct Mol Biol. 2008 Feb;15(2):130-8. Epub 2008 Jan 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18204465 18204465]
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== Function ==
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[[Category: Escherichia coli]]
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[https://www.uniprot.org/uniprot/Q8XBV8_ECO57 Q8XBV8_ECO57]
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Cygler, M.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Matte, A.]]
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Check<jmol>
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[[Category: Tocilj, A.]]
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<jmolCheckbox>
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[[Category: bacterial polysaccharide co-polymerase]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b8/3b8n_consurf.spt"</scriptWhenChecked>
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[[Category: biosynthetic protein]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: fepe]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: metal transport]]
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</jmolCheckbox>
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[[Category: wzz]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b8n ConSurf].
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<div style="clear:both"></div>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:04:11 2008''
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli O157:H7]]
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[[Category: Large Structures]]
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[[Category: Cygler M]]
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[[Category: Matte A]]
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[[Category: Tocilj A]]

Current revision

Structure of FepE- Bacterial Polysaccharide Co-polymerase

PDB ID 3b8n

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