3beo

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(New page: 200px<br /><applet load="3beo" size="350" color="white" frame="true" align="right" spinBox="true" caption="3beo, resolution 1.700&Aring;" /> '''A Structural Basis ...)
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[[Image:3beo.jpg|left|200px]]<br /><applet load="3beo" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="3beo, resolution 1.700&Aring;" />
 
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'''A Structural Basis for the allosteric regulation of non-hydrolyzing UDP-GlcNAc 2-epimerases'''<br />
 
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==Overview==
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==A Structural Basis for the allosteric regulation of non-hydrolyzing UDP-GlcNAc 2-epimerases==
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The non-hydrolysing bacterial UDP-N-acetylglucosamine 2-epimerase (UDP-GlcNAc 2-epimerase) catalyses the conversion of UDP-GlcNAc into UDP-N-acetylmannosamine, an intermediate in the biosynthesis of several cell-surface polysaccharides. This enzyme is allosterically regulated by its substrate UDP-GlcNAc. The structure of the ternary complex between the Bacillus anthracis UDP-GlcNAc 2-epimerase, its substrate UDP-GlcNAc and the reaction intermediate UDP, showed direct interactions between UDP and its substrate, and between the complex and highly conserved enzyme residues, identifying the allosteric site of the enzyme. The binding of UDP-GlcNAc is associated with conformational changes in the active site of the enzyme. Kinetic data and mutagenesis of the highly conserved UDP-GlcNAc-interacting residues confirm their importance in the substrate binding and catalysis of the enzyme. This constitutes the first example to our knowledge, of an enzymatic allosteric activation by direct interaction between the substrate and the allosteric activator.
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<StructureSection load='3beo' size='340' side='right'caption='[[3beo]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[3beo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BEO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BEO FirstGlance]. <br>
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3BEO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] with <scene name='pdbligand=UD1:'>UD1</scene> and <scene name='pdbligand=UDP:'>UDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_2-epimerase UDP-N-acetylglucosamine 2-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.14 5.1.3.14] Known structural/functional Sites: <scene name='pdbsite=AC1:Ud1+Binding+Site+For+Residue+A+372'>AC1</scene>, <scene name='pdbsite=AC2:Ud1+Binding+Site+For+Residue+B+372'>AC2</scene>, <scene name='pdbsite=AC3:Udp+Binding+Site+For+Residue+A+1081'>AC3</scene> and <scene name='pdbsite=AC4:Udp+Binding+Site+For+Residue+B+1081'>AC4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BEO OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3beo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3beo OCA], [https://pdbe.org/3beo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3beo RCSB], [https://www.ebi.ac.uk/pdbsum/3beo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3beo ProSAT]</span></td></tr>
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A structural basis for the allosteric regulation of non-hydrolysing UDP-GlcNAc 2-epimerases., Velloso LM, Bhaskaran SS, Schuch R, Fischetti VA, Stebbins CE, EMBO Rep. 2008 Feb;9(2):199-205. Epub 2008 Jan 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18188181 18188181]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A348ACF3_BACAN A0A348ACF3_BACAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/3beo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3beo ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus anthracis]]
[[Category: Bacillus anthracis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: UDP-N-acetylglucosamine 2-epimerase]]
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[[Category: Bhaskaran SS]]
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[[Category: Bhaskaran, S S.]]
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[[Category: Fischetti VA]]
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[[Category: Fischetti, V A.]]
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[[Category: Schuch R]]
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[[Category: Schuch, R.]]
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[[Category: Stebbins CE]]
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[[Category: Stebbins, C E.]]
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[[Category: Velloso LM]]
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[[Category: Velloso, L M.]]
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[[Category: UD1]]
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[[Category: UDP]]
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[[Category: allosteric]]
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[[Category: epimerase]]
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[[Category: isomerase]]
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[[Category: regulation]]
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[[Category: udp-glcnac]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:05:15 2008''
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Current revision

A Structural Basis for the allosteric regulation of non-hydrolyzing UDP-GlcNAc 2-epimerases

PDB ID 3beo

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