3ir1

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{{STRUCTURE_3ir1| PDB=3ir1 | SCENE= }}
 
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===Crystal Structure of Lipoprotein GNA1946 from Neisseria meningitidis===
 
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{{ABSTRACT_PUBMED_19733245}}
 
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==About this Structure==
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==Crystal Structure of Lipoprotein GNA1946 from Neisseria meningitidis==
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[[3ir1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplokokkus_intracellularis_meningitidis"_(sic)_weichselbaum_1887 "diplokokkus intracellularis meningitidis" (sic) weichselbaum 1887]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IR1 OCA].
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<StructureSection load='3ir1' size='340' side='right'caption='[[3ir1]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ir1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis Neisseria meningitidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IR1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ir1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ir1 OCA], [https://pdbe.org/3ir1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ir1 RCSB], [https://www.ebi.ac.uk/pdbsum/3ir1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ir1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7BMQ8_NEIME Q7BMQ8_NEIME]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ir/3ir1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ir1 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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GNA1946, a conserved outer membrane lipoprotein from Neisseria meningitidis, has been identified as a candidate antigen for an urgently needed broad-spectrum meningococcal vaccine. It has been predicted to be a periplasmic receptor in the D-methionine uptake ABC transporter system. The crystal structure of GNA1946 was solved by the single-wavelength anomalous dispersion (SAD) method to a resolution of 2.25 A, and it reveals a Venus flytrap-like structure. GNA1946 consists of two globular lobes connected by a hinge region. Surprisingly, the structure showed an L-methionine bound within the cleft between the lobes. A comparison of GNA1946 with two other outer membrane lipoproteins, the L-methionine-binding Tp32 from Treponema pallidum and the dipeptide GlyMet-binding protein Pg110 from Staphylococcus aureus, revealed that although these three proteins share low sequence similarities, there is a high degree of structural conservation and similar substrate-binding frameworks. Our results reveal that GNA1946 is an L-methionine binding lipoprotein in the outer membrane, and should function as an initial receptor for ABC transporters with high affinity and specificity. The GNA1946 structure reported here should provide a valuable starting point for the development of a broad-spectrum meningococcal vaccine.
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==Reference==
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Crystal structure of lipoprotein GNA1946 from Neisseria meningitidis.,Yang X, Wu Z, Wang X, Yang C, Xu H, Shen Y J Struct Biol. 2009 Dec;168(3):437-43. Epub 2009 Sep 3. PMID:19733245<ref>PMID:19733245</ref>
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<ref group="xtra">PMID:019733245</ref><references group="xtra"/><references/>
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[[Category: Shen, Y.]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Wang, X.]]
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</div>
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[[Category: Wu, Z.]]
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<div class="pdbe-citations 3ir1" style="background-color:#fffaf0;"></div>
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[[Category: Yang, X.]]
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== References ==
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[[Category: D-methionine cultured]]
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<references/>
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[[Category: Gna1946]]
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__TOC__
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[[Category: Lipoprotein]]
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</StructureSection>
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[[Category: Protein binding]]
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[[Category: Large Structures]]
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[[Category: Neisseria meningitidis]]
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[[Category: Shen Y]]
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[[Category: Wang X]]
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[[Category: Wu Z]]
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[[Category: Yang X]]

Current revision

Crystal Structure of Lipoprotein GNA1946 from Neisseria meningitidis

PDB ID 3ir1

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