4oou
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4oou is ON HOLD Authors: Kim, M.-K., An, Y.J., Jeong, C.-S., Cha, S.-S. Description: Crystal structure of beta-1,4-D-mannanase from Cryptopygus ant...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of beta-1,4-D-mannanase from Cryptopygus antarcticus== | |
| + | <StructureSection load='4oou' size='340' side='right'caption='[[4oou]], [[Resolution|resolution]] 2.36Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4oou]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptopygus_antarcticus Cryptopygus antarcticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OOU FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.36Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oou OCA], [https://pdbe.org/4oou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oou RCSB], [https://www.ebi.ac.uk/pdbsum/4oou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oou ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MANA_CRYAT MANA_CRYAT] Hydrolyzes 1,4-beta linked polysaccharide backbones of mannans. Has high activity toward locust bean gum (PubMed:18579426, PubMed:25082572). Also active toward konjac and beta-1,4-mannan. Hydrolyzes mannotetraose (M4) and mannopentaose (M5) to mannobiose (M2) and mannotriose (M3) with a little production of mannose (M1). Hydrolyzes beta-1,4-mannan to M2, M3 and M4. Hardly hydrolyzes M2 and M3. Does not hydrolyze p-nitrophenyl-beta-D-mannopyranoside, gua-gum, carboxymethyl cellulose, soluble starch or laminarin (PubMed:18579426).<ref>PMID:18579426</ref> <ref>PMID:25082572</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Endo-beta-1,4-d-mannanase from the Antarctic springtail, Cryptopygus antarcticus (CaMan), is a cold-adapted beta-mannanase that has the lowest optimum temperature (30 degrees C) of all known beta-mannanases. Here, we report the apo- and mannopentaose (M5) complex structures of CaMan. Structural comparison of CaMan with other beta-mannanases from the multicellular animals reveals that CaMan has an extended loop that alters topography of the active site. Structural and mutational analyses suggest that this extended loop is linked to the cold-adapted enzymatic activity. From the CaMan-M5 complex structure, we defined the mannose-recognition subsites and observed unreported M5 binding site on the surface of CaMan. Proteins 2014; 82:3217-3223. (c) 2014 Wiley Periodicals, Inc. | ||
| - | + | Structure-based investigation into the functional roles of the extended loop and substrate-recognition sites in an endo-beta-1,4-d-mannanase from the Antarctic springtail, Cryptopygus antarcticus.,Kim MK, An YJ, Song JM, Jeong CS, Kang MH, Kwon KK, Lee YH, Cha SS Proteins. 2014 Nov;82(11):3217-23. doi: 10.1002/prot.24655. Epub 2014 Aug 19. PMID:25082572<ref>PMID:25082572</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4oou" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cryptopygus antarcticus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: An YJ]] | ||
| + | [[Category: Cha S-S]] | ||
| + | [[Category: Jeong C-S]] | ||
| + | [[Category: Kim M-K]] | ||
Current revision
Crystal structure of beta-1,4-D-mannanase from Cryptopygus antarcticus
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