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3byh

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(New page: 200px<br /><applet load="3byh" size="350" color="white" frame="true" align="right" spinBox="true" caption="3byh" /> '''Model of actin-fimbrin ABD2 complex'''<br />...)
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[[Image:3byh.jpg|left|200px]]<br /><applet load="3byh" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="3byh" />
 
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'''Model of actin-fimbrin ABD2 complex'''<br />
 
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==Overview==
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==Model of actin-fimbrin ABD2 complex==
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Many actin binding proteins have a modular architecture, and calponin-homology (CH) domains are one such structurally conserved module found in numerous proteins that interact with F-actin. The manner in which CH-domains bind F-actin has been controversial. Using cryo-EM and a single-particle approach to helical reconstruction, we have generated 12-A-resolution maps of F-actin alone and F-actin decorated with a fragment of human fimbrin (L-plastin) containing tandem CH-domains. The high resolution allows an unambiguous fit of the crystal structure of fimbrin into the map. The interaction between fimbrin ABD2 (actin binding domain 2) and F-actin is different from any interaction previously observed or proposed for tandem CH-domain proteins, showing that the structural conservation of the CH-domains does not lead to a conserved mode of interaction with F-actin. Both the stapling of adjacent actin protomers and the additional closure of the nucleotide binding cleft in F-actin when the fimbrin fragment binds may explain how fimbrin can stabilize actin filaments. A mechanism is proposed where ABD1 of fimbrin becomes activated for binding a second actin filament after ABD2 is bound to a first filament, and this can explain how mutations of residues buried in the interface between ABD2 and ABD1 can rescue temperature-sensitive defects in actin.
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<SX load='3byh' size='340' side='right' viewer='molstar' caption='[[3byh]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3byh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BYH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3byh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3byh OCA], [https://pdbe.org/3byh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3byh RCSB], [https://www.ebi.ac.uk/pdbsum/3byh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3byh ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/ACTB_HUMAN ACTB_HUMAN] Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:[https://omim.org/entry/607371 607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.<ref>PMID:16685646</ref> Defects in ACTB are the cause of Baraitser-Winter syndrome type 1 (BRWS1) [MIM:[https://omim.org/entry/243310 243310]. A rare developmental disorder characterized by the combination of congenital ptosis, high-arched eyebrows, hypertelorism, ocular colobomata, and a brain malformation consisting of anterior-predominant lissencephaly. Other typical features include postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss.<ref>PMID:22366783</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/ACTB_HUMAN ACTB_HUMAN] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/by/3byh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3byh ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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3BYH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BYH OCA].
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*[[Actin 3D structures|Actin 3D structures]]
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== References ==
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==Reference==
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<references/>
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High-resolution cryo-EM structure of the F-actin-fimbrin/plastin ABD2 complex., Galkin VE, Orlova A, Cherepanova O, Lebart MC, Egelman EH, Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1494-8. Epub 2008 Jan 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18234857 18234857]
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__TOC__
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</SX>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Cherepanova, O.]]
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[[Category: Cherepanova O]]
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[[Category: Egelman, E H.]]
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[[Category: Egelman EH]]
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[[Category: Galkin, V E.]]
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[[Category: Galkin VE]]
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[[Category: Lebart, M C.]]
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[[Category: Lebart MC]]
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[[Category: Orlova, A.]]
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[[Category: Orlova A]]
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[[Category: acetylation]]
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[[Category: atp-binding]]
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[[Category: cytoplasm]]
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[[Category: cytoskeleton]]
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[[Category: helical filament]]
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[[Category: methylation]]
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[[Category: nucleotide-binding]]
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[[Category: phosphoprotein]]
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[[Category: protein polymer]]
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[[Category: structural protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:07:55 2008''
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Current revision

Model of actin-fimbrin ABD2 complex

3byh, resolution 12.00Å

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