4nod

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{{STRUCTURE_4nod| PDB=4nod | SCENE= }}
 
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===Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation===
 
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{{ABSTRACT_PUBMED_24435062}}
 
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==Function==
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==Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation==
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[[http://www.uniprot.org/uniprot/TFAM_HUMAN TFAM_HUMAN]] Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.<ref>PMID:1737790</ref> <ref>PMID:20410300</ref> <ref>PMID:19304746</ref> <ref>PMID:22037172</ref> <ref>PMID:22037171</ref>
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<StructureSection load='4nod' size='340' side='right'caption='[[4nod]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4nod]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NOD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.897&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nod OCA], [https://pdbe.org/4nod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nod RCSB], [https://www.ebi.ac.uk/pdbsum/4nod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nod ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TFAM_HUMAN TFAM_HUMAN] Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.<ref>PMID:1737790</ref> <ref>PMID:20410300</ref> <ref>PMID:19304746</ref> <ref>PMID:22037172</ref> <ref>PMID:22037171</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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TFAM (transcription factor A, mitochondrial) is a DNA-binding protein that activates transcription at the two major promoters of mitochondrial DNA (mtDNA)-the light strand promoter (LSP) and the heavy strand promoter 1 (HSP1). Equally important, it coats and packages the mitochondrial genome. TFAM has been shown to impose a U-turn on LSP DNA; however, whether this distortion is relevant at other sites is unknown. Here we present crystal structures of TFAM bound to HSP1 and to nonspecific DNA. In both, TFAM similarly distorts the DNA into a U-turn. Yet, TFAM binds to HSP1 in the opposite orientation from LSP explaining why transcription from LSP requires DNA bending, whereas transcription at HSP1 does not. Moreover, the crystal structures reveal dimerization of DNA-bound TFAM. This dimerization is dispensable for DNA bending and transcriptional activation but is important in DNA compaction. We propose that TFAM dimerization enhances mitochondrial DNA compaction by promoting looping of the DNA.
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==About this Structure==
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Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation.,Ngo HB, Lovely GA, Phillips R, Chan DC Nat Commun. 2014 Jan 17;5:3077. doi: 10.1038/ncomms4077. PMID:24435062<ref>PMID:24435062</ref>
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[[4nod]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NOD OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024435062</ref><references group="xtra"/><references/>
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</div>
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[[Category: Chan, D C.]]
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<div class="pdbe-citations 4nod" style="background-color:#fffaf0;"></div>
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[[Category: Lovely, G A.]]
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== References ==
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[[Category: Ngo, H B.]]
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<references/>
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[[Category: Phillips, R.]]
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__TOC__
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[[Category: Bru substitution to hsp1]]
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</StructureSection>
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[[Category: Dna bending]]
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[[Category: Homo sapiens]]
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[[Category: Hmg]]
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[[Category: Large Structures]]
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[[Category: Mtrnap]]
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[[Category: Chan DC]]
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[[Category: T19]]
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[[Category: Lovely GA]]
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[[Category: Tfb2m]]
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[[Category: Ngo HB]]
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[[Category: Transcription regulator-dna complex]]
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[[Category: Phillips R]]

Current revision

Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation

PDB ID 4nod

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