3kvt

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[[Image:3kvt.jpg|left|200px]]<br /><applet load="3kvt" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="3kvt, resolution 2.00&Aring;" />
 
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'''TETRAMERIZATION DOMAIN FROM AKV3.1 (SHAW-SUBFAMILY) VOLTAGE-GATED POTASSIUM CHANNEL'''<br />
 
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==Overview==
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==TETRAMERIZATION DOMAIN FROM AKV3.1 (SHAW-SUBFAMILY) VOLTAGE-GATED POTASSIUM CHANNEL==
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The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated K+ channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. Crystal structures of T1 tetramers from Shaw and Shaker subfamilies reveal a common four-layered scaffolding. Within layer 4, on the hypothetical membrane-facing side of the tetramer, the Shaw T1 tetramer contains four zinc ions; each is coordinated by a histidine and two cysteines from one monomer and by one cysteine from an adjacent monomer. The amino acids involved in coordinating the Zn2+ ion occur in a HX5CX20CC sequence motif that is highly conserved among all Shab, Shaw and Shal subfamily members, but is not found in Shaker subfamily members. We demonstrate by coimmunoprecipitation that a few characteristic residues in the subunit interface are crucial for subfamily-specific tetramerization of the T1 domains.
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<StructureSection load='3kvt' size='340' side='right'caption='[[3kvt]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3kvt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KVT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kvt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kvt OCA], [https://pdbe.org/3kvt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kvt RCSB], [https://www.ebi.ac.uk/pdbsum/3kvt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kvt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O76457_APLCA O76457_APLCA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kv/3kvt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kvt ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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3KVT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVT OCA].
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*[[Potassium channel 3D structures|Potassium channel 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels., Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S, Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9886290 9886290]
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[[Category: Aplysia californica]]
[[Category: Aplysia californica]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bellamy, H.]]
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[[Category: Bellamy H]]
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[[Category: Bixby, K A.]]
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[[Category: Bixby KA]]
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[[Category: Choe, S.]]
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[[Category: Choe S]]
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[[Category: Kreusch, A.]]
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[[Category: Kreusch A]]
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[[Category: Nanao, M H.]]
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[[Category: Nanao MH]]
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[[Category: Pfaffinger, P J.]]
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[[Category: Pfaffinger PJ]]
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[[Category: Shen, N V.]]
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[[Category: Shen NV]]
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[[Category: ZN]]
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[[Category: molecular recognition]]
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[[Category: potassium channel]]
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[[Category: tetramerization domain]]
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[[Category: zinc-binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:10:01 2008''
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Current revision

TETRAMERIZATION DOMAIN FROM AKV3.1 (SHAW-SUBFAMILY) VOLTAGE-GATED POTASSIUM CHANNEL

PDB ID 3kvt

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