This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4jkn

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:44, 20 September 2023) (edit) (undo)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_4jkn| PDB=4jkn | SCENE= }}
 
-
===Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A===
 
-
==Function==
+
==Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A==
-
[[http://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.
+
<StructureSection load='4jkn' size='340' side='right'caption='[[4jkn]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[4jkn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JKN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JKN FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.536&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jkn OCA], [https://pdbe.org/4jkn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jkn RCSB], [https://www.ebi.ac.uk/pdbsum/4jkn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jkn ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase.
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Mercury(II) metallation of Pseudomonas aeruginosa azurin has been characterized structurally and biochemically. The X-ray crystal structure at 1.5A of mercury(II) metallated azurin confirms the coordination of mercury at the copper binding active site and a second surface site. These findings are further validated by NMR, Matrix-assisted laser desorption/ionization spectrometry (MALDI), and UV-visible spectroscopic methods indicating copper displacement from the wild-type protein. Bioinformatic analysis has identified homologous human protein domains computationally, and compared them to the structure of azurin, providing a model for human mercury interactions. Study of the mercury-azurin adduct, in combination with other known examples of protein-heavy metal interactions, could provide further insight into the chemical mechanisms of toxicological interactions, leading toward a global understanding of the biological speciation of toxic heavy metals.
-
==About this Structure==
+
Mercury metallation of the copper protein azurin and structural insight into possible heavy metal reactivity.,Zampino AP, Masters FM, Bladholm EL, Panzner MJ, Berry SM, Leeper TC, Ziegler CJ J Inorg Biochem. 2014 Dec;141:152-60. doi: 10.1016/j.jinorgbio.2014.09.003. Epub , 2014 Sep 16. PMID:25265377<ref>PMID:25265377</ref>
-
[[4jkn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JKN OCA].
+
 
-
[[Category: Berry, S B.]]
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Bladholm, E L.]]
+
</div>
-
[[Category: Masters, F M.]]
+
<div class="pdbe-citations 4jkn" style="background-color:#fffaf0;"></div>
-
[[Category: Panzner, M J.]]
+
 
-
[[Category: Zampino, A P.]]
+
==See Also==
-
[[Category: Ziegler, C J.]]
+
*[[Azurin 3D structures|Azurin 3D structures]]
-
[[Category: Electron transport]]
+
== References ==
-
[[Category: Mercury metallation]]
+
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Pseudomonas aeruginosa PAO1]]
 +
[[Category: Berry SB]]
 +
[[Category: Bladholm EL]]
 +
[[Category: Masters FM]]
 +
[[Category: Panzner MJ]]
 +
[[Category: Zampino AP]]
 +
[[Category: Ziegler CJ]]

Current revision

Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A

PDB ID 4jkn

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4jkn"
Personal tools