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Galactose-binding lectin

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<StructureSection load='1v6i' size='350' side='right' caption='Structure of peanut galactose-binding lectin tetramer complex with β-D-galactose, α-D-glucose, Ca+2 (green) and Mn+2 (purple) ions (PDB entry [[1v6i]])' scene=''>
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<StructureSection load='' size='450' side='right' caption='Structure of peanut galactose-binding lectin tetramer complex with β-D-galactose, α-D-glucose, Ca+2 (green) and Mn+2 (purple) ions (PDB entry [[1v6i]])' scene='57/572274/Cv/1'>
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== Function ==
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'''Galactose-binding lectin''' (GBL) is a lectin which binds the carbohydrate sequence Gal-β(1,3)-GalNac. GBL is an all beta-sheet protein. GBL is used in affinity columns for capturing proteins which are glycosylated with Gal-β(1,3)-GalNac.
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'''Galactose-binding lectin''' (GBL) is a lectin which binds the carbohydrate sequence Gal-β(1,3)-GalNac. GBL is an all beta-sheet protein<ref>PMID:17057347</ref>.
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== Relevance ==
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GBL is used in affinity columns for capturing proteins which are glycosylated with Gal-β(1,3)-GalNac.
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== Structural highlights ==
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The carbohydrate ligand binds to 3 loops<ref>PMID:14747696</ref>. <scene name='57/572274/Cv/6'>β-D-galactose/α-D-glucose binding site</scene> in peanut galactose-binding lectin (PDB entry [[1v6i]]).
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<scene name='57/572274/Cv/7'>Ca coordination site</scene>.
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<scene name='57/572274/Cv/8'>Mn coordination site</scene>. Water molecules shown as red spheres.
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</StructureSection>
== 3D structures of galactose-binding lectin ==
== 3D structures of galactose-binding lectin ==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Peanut GBL
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**[[1cq9]], [[1v6m]], [[1v6n]], [[1v6o]], [[2dh1]], [[2dvf]] – GBL <br />
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'''Peanut GBL'''
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*''Peanut GBL binary complex''
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[[1cq9]], [[1v6m]], [[1v6n]], [[1v6o]], [[2dh1]], [[2dvf]] – GBL <br />
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**[[1qf3]] – GBL + methyl-β-galactose <br />
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**[[2dv9]], [[2dvd]] – GBL + β-D-galactose <br />
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**[[1rir]] – GBL + porphyrin derivative <br />
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**[[6v95]], [[6vav]], [[6vaw]], [[6vc3]], [[6vc4]], [[6vgf]] – GBL + galactopyranose derivative <br />
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''Peanut GBL binary complex''
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*''Peanut GBL ternary complex''
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[[1qf3]] – GBL + methyl-β-galactose <br />
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**[[1v6i]], [[1v6j]], [[1v6k]], [[1cr7]], [[2dvg]] – GBL + β-D-galactose + α-D-glucose <br />
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[[2dv9]], [[2dvd]] – GBL + β-D-galactose <br />
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**[[2pel]] – GBL + β-lactose + α-lactose <br />
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[[1rir]] – GBL + porphyrin derivative <br />
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**[[2tep]], [[2dva]], [[2dvb]] – GBL + β-D-galactose + N-acetyl-D-galactosamine <br />
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**[[1ciw]] – GBL + β-D-galactose + 2-acetylamino-2-deoxy-D-glucopyranose <br />
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**[[1rit]] – GBL + porphyrin derivative + β-lactose<br />
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''Peanut GBL ternary complex''
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*''Peanut GBL quaternary complex''
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[[1v6i]], [[1v6j]], [[1v6k]], [[1cr7]], [[2dvg]] – GBL + β-D-galactose + α-D-glucose <br />
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**[[1bzw]], [[1v6l]] – GBL + β-D-galactose + α-D-glucose + β-D-glucose<br />
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[[2pel]] – GBL + β-lactose + α-lactose <br />
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[[2tep]], [[2dva]], [[2dvb]] – GBL + β-D-galactose + N-acetyl-D-galactosamine <br />
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[[1ciw]] – GBL + β-D-galactose + 2-acetylamino-2-deoxy-D-glucopyranose <br />
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[[1rit]] – GBL + porphyrin derivative + β-lactose<br />
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''Peanut GBL quaternary complex''
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*''Pseudomonas aeruginosa'' GBL
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[[1bzw]], [[1v6l]] – GBL + β-D-galactose + α-D-glucose + β-D-glucose<br />
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**[[1l7l]], [[1uoj]] – GBL <br />
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**[[4cp9]], [[4cpb]], [[7z62]], [[7z63]] – GBL + galactoside <br />
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**[[7fio]], [[7fjh]] – GBL + inhibitor<br />
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'''''Pseudomonas aeruginosa'' GBL'''
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*''PaGBL binary complex''
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[[1l7l]], [[1uoj]] – GBL <br />
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**[[2wyf]] – GBL + methyl-β-galactose <br />
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**[[4ljh]] – GBL + β-D-galactose <br />
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**[[3zyf]], [[4a6s]], [[4yw6]], [[5mih]] – GBL + galactopyranose derivative<br />
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**[[4ywa]] – GBL + triazole derivative <br />
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**[[6yo3]], [[6yoh]] – GBL + oxidanyl derivative <br />
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''PsGBL binary complex''
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*''PaGBL ternary complex''
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[[2wyf]] – GBL + methyl-β-galactose <br />
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**[[3zyh]] – GBL + galactopyranose derivative + gal-lys-pro-leu <br />
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[[4ljh]] – GBL + β-D-galactose <br />
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**[[4yw7]] – GBL + galactopyranose derivative + triazole derivative <br />
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[[3zyf]], [[4a6s]] – GBL + galactopyranose derivative<br />
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**[[3zyb]] – GBL + β-D-galactose + gal-lys-pro-leu<br />
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**[[4lk6]] – GBL + β-D-galactose + chlorophenol red<br />
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**[[4lk7]] – GBL + β-D-galactose + resorufin<br />
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**[[1oko]] – GBL + β-D-galactose + α-D-galactose <br />
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**[[4al9]] – GBL + α-D-galactose + α-D-glucose <br />
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''PsGBL ternary complex''
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*''PaGBL quaternary complex''
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[[3zyh]] – GBL + galactopyranose derivative + gal-lys-pro-leu <br />
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**[[2vxj]] – GBL + β-D-galactose + α-D-galactose + α-D-glucose + β-D-glucose<br />
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[[3zyb]] – GBL + β-D-galactose + gal-lys-pro-leu<br />
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[[4lk6]] – GBL + β-D-galactose + chlorophenol red<br />
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[[4lk7]] – GBL + β-D-galactose + resorufin<br />
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[[1oko]] – GBL + β-D-galactose + α-D-galactose <br />
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[[4al9]] – GBL + α-D-galactose + α-D-glucose <br />
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''PsGBL quaternary complex''
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*''Lumbricus terrestris'' GBL
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[[2vxj]] – GBL + β-D-galactose + α-D-galactose + α-D-glucose + β-D-glucose<br />
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**[[2dry]] – LtGBL C terminal (mutant) – ''Lumbricus terrestris''<br />
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**[[2rst]] – LtGBL C terminal <br />
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**[[2drz]] – LtGBL C terminal (mutant) + lactose <br />
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**[[2zqn]] – LtGBL C terminal + lactose <br />
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**[[2ds0]] – LtGBL C terminal (mutant) + sialyllactose <br />
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**[[2zqo]] – LtGBL C terminal + GalNac <br />
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[[2dry]] – LtGBL C terminal (mutant) – ''Lumbricus terrestris''<br />
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*Other GBL
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[[2rst]] – LtGBL C terminal <br />
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[[2drz]] – LtGBL C terminal (mutant) + lactose <br />
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[[2zqn]] – LtGBL C terminal + lactose <br />
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[[2ds0]] – LtGBL C terminal (mutant) + sialyllactose <br />
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[[2zqo]] – LtGBL C terminal + GalNac <br />
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**[[5x4a]], [[3wmp]], [[3wmq]] – GBL residues 47-140 + polysaccharide – ''Sinularia lochmodes'' <br />
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**[[2zqn]] – eGBL C-terminal + lactose – earthworm<br />
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**[[2zqo]] – eGBL C-terminal + GalNac<br />
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**[[6yf6]] – GBL C-terminal + fucoside derivative – ''Enterobacter cloacae''<br />
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**[[5vbk]], [[6bfm]] – GBL - mussell<br />
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}}
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Structure of peanut galactose-binding lectin tetramer complex with β-D-galactose, α-D-glucose, Ca+2 (green) and Mn+2 (purple) ions (PDB entry 1v6i)

Drag the structure with the mouse to rotate

3D structures of galactose-binding lectin

Updated on 07-March-2023

References

  1. ↑ Natchiar SK, Srinivas O, Mitra N, Surolia A, Jayaraman N, Vijayan M. Structural studies on peanut lectin complexed with disaccharides involving different linkages: further insights into the structure and interactions of the lectin. Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1413-21. Epub 2006, Oct 18. PMID:17057347 doi:10.1107/S0907444906035712
  2. ↑ Kundhavai Natchiar S, Arockia Jeyaprakash A, Ramya TN, Thomas CJ, Suguna K, Surolia A, Vijayan M. Structural plasticity of peanut lectin: an X-ray analysis involving variation in pH, ligand binding and crystal structure. Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):211-9. Epub 2004, Jan 23. PMID:14747696 doi:http://dx.doi.org/10.1107/S090744490302849X

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Michal Harel, Alexander Berchansky, Joel L. Sussman

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