4p2v
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4p2v is ON HOLD Authors: Miller, S.T., Oh, I.K., Xavier, K.B. Description: Structure of the AI-2 processing enzyme LsrF in complex with the product...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the AI-2 processing enzyme LsrF in complex with the product of the LsrG reaction P-HPD== | |
| + | <StructureSection load='4p2v' size='340' side='right'caption='[[4p2v]], [[Resolution|resolution]] 2.51Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4p2v]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P2V FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=26T:(3R)-3-HYDROXY-2,4-DIOXOPENTYL+DIHYDROGEN+PHOSPHATE'>26T</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p2v OCA], [https://pdbe.org/4p2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p2v RCSB], [https://www.ebi.ac.uk/pdbsum/4p2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p2v ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LSRF_ECOLI LSRF_ECOLI] Could be involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The quorum sensing signal autoinducer-2 (AI-2) regulates important bacterial behaviors, including biofilm formation and the production of virulence factors. Some bacteria, such as Escherichia coli, can quench the AI-2 signal produced by a variety of species present in the environment, and thus can influence AI-2-dependent bacterial behaviors. This process involves uptake of AI-2 via the Lsr transporter, followed by phosphorylation and consequent intracellular sequestration. Here we determine the metabolic fate of intracellular AI-2 by characterizing LsrF, the terminal protein in the Lsr AI-2 processing pathway. We identify the substrates of LsrF as 3-hydroxy-2,4-pentadione-5-phosphate (P-HPD, an isomer of AI-2-phosphate) and coenzyme A, determine the crystal structure of an LsrF catalytic mutant bound to P-HPD, and identify the reaction products. We show that LsrF catalyzes the transfer of an acetyl group from P-HPD to coenzyme A yielding dihydroxyacetone phosphate and acetyl-CoA, two key central metabolites. We further propose that LsrF, despite strong structural homology to aldolases, acts as a thiolase, an activity previously undescribed for this family of enzymes. With this work, we have fully characterized the biological pathway for AI-2 processing in E. coli, a pathway that can be used to quench AI-2 and control quorum-sensing-regulated bacterial behaviors. | ||
| - | + | LsrF, a coenzyme A-dependent thiolase, catalyzes the terminal step in processing the quorum sensing signal autoinducer-2.,Marques JC, Oh IK, Ly DC, Lamosa P, Ventura MR, Miller ST, Xavier KB Proc Natl Acad Sci U S A. 2014 Sep 15. pii: 201408691. PMID:25225400<ref>PMID:25225400</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4p2v" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Aldolase 3D structures|Aldolase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia coli K-12]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Miller ST]] | ||
| + | [[Category: Oh IK]] | ||
| + | [[Category: Xavier KB]] | ||
Current revision
Structure of the AI-2 processing enzyme LsrF in complex with the product of the LsrG reaction P-HPD
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