4nf6

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{{STRUCTURE_4nf6| PDB=4nf6 | SCENE= }}
 
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===Crystal structure of GluN1/GluN2A ligand-binding domain in complex with glycine and PPDA===
 
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{{ABSTRACT_PUBMED_24462099}}
 
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==Function==
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==Crystal structure of GluN1/GluN2A ligand-binding domain in complex with glycine and PPDA==
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[[http://www.uniprot.org/uniprot/NMDZ1_RAT NMDZ1_RAT]] NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.<ref>PMID:15996549</ref> [[http://www.uniprot.org/uniprot/NMDE1_RAT NMDE1_RAT]] NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
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<StructureSection load='4nf6' size='340' side='right'caption='[[4nf6]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4nf6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NF6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NF6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2JL:(2S,3R)-1-(PHENANTHREN-2-YLCARBONYL)PIPERAZINE-2,3-DICARBOXYLIC+ACID'>2JL</scene>, <scene name='pdbligand=GLY:GLYCINE'>GLY</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nf6 OCA], [https://pdbe.org/4nf6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nf6 RCSB], [https://www.ebi.ac.uk/pdbsum/4nf6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nf6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NMDZ1_RAT NMDZ1_RAT] NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.<ref>PMID:15996549</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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There has been a great level of enthusiasm to downregulate overactive N-methyl-D-aspartate (NMDA) receptors to protect neurons from excitotoxicity. NMDA receptors play pivotal roles in basic brain development and functions as well as in neurological disorders and diseases. However, mechanistic understanding of antagonism in NMDA receptors is limited due to complete lack of antagonist-bound structures for the L-glutamate-binding GluN2 subunits. Here, we report the crystal structures of GluN1/GluN2A NMDA receptor ligand-binding domain (LBD) heterodimers in complex with GluN1- and GluN2-targeting antagonists. The crystal structures reveal that the antagonists, D-(-)-2-amino-5-phosphonopentanoic acid (D-AP5) and 1-(phenanthrene-2-carbonyl)piperazine-2,3-dicarboxylic acid (PPDA), have discrete binding modes and mechanisms for opening of the bilobed architecture of GluN2A LBD compared to the agonist-bound form. The current study shows distinct ways by which the conformations of NMDA receptor LBDs may be controlled and coupled to receptor inhibition and provides possible strategies to develop therapeutic compounds with higher subtype-specificity.
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==About this Structure==
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Structural insights into competitive antagonism in NMDA receptors.,Jespersen A, Tajima N, Fernandez-Cuervo G, Garnier-Amblard EC, Furukawa H Neuron. 2014 Jan 22;81(2):366-78. doi: 10.1016/j.neuron.2013.11.033. PMID:24462099<ref>PMID:24462099</ref>
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[[4nf6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NF6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024462099</ref><references group="xtra"/><references/>
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</div>
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[[Category: Furukawa, H.]]
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<div class="pdbe-citations 4nf6" style="background-color:#fffaf0;"></div>
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[[Category: Jespersen, A.]]
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[[Category: Tajima, N.]]
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==See Also==
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[[Category: Glycine and ppda]]
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*[[Glutamate receptor 3D structures|Glutamate receptor 3D structures]]
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[[Category: Receptor]]
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== References ==
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[[Category: Transport protein]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Rattus norvegicus]]
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[[Category: Furukawa H]]
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[[Category: Jespersen A]]
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[[Category: Tajima N]]

Current revision

Crystal structure of GluN1/GluN2A ligand-binding domain in complex with glycine and PPDA

PDB ID 4nf6

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