4jj2

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{{STRUCTURE_4jj2| PDB=4jj2 | SCENE= }}
 
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===High resolution structure of a C-terminal fragment of the T4 phage gp5 beta-helix===
 
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==Function==
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==High resolution structure of a C-terminal fragment of the T4 phage gp5 beta-helix==
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[[http://www.uniprot.org/uniprot/VG05_BPT4 VG05_BPT4]] Tail-associated lysozyme essential for localized hydrolysis of bacterial cell wall necessary for viral DNA injection. The needle-like gp5 protein punctures the outer cell membrane and then digests the peptidoglycan cell wall in the periplasmic space.
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<StructureSection load='4jj2' size='340' side='right'caption='[[4jj2]], [[Resolution|resolution]] 1.28&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4jj2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JJ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JJ2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.28&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ELA:9-OCTADECENOIC+ACID'>ELA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=STE:STEARIC+ACID'>STE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jj2 OCA], [https://pdbe.org/4jj2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jj2 RCSB], [https://www.ebi.ac.uk/pdbsum/4jj2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jj2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NEEDL_BPT4 NEEDL_BPT4] Tail-associated lysozyme of the baseplate hub that is essential for localized hydrolysis of bacterial cell wall necessary for viral DNA injection. The needle-like gp5 protein punctures the outer cell membrane and then digests the peptidoglycan cell wall in the periplasmic space. Involved in the tail assembly.<ref>PMID:12837775</ref> <ref>PMID:21129200</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Gene product 5 (gp5) of bacteriophage T4 is a spike-shaped protein that functions to disrupt the membrane of the target cell during phage infection. Its C-terminal domain is a long and slender beta-helix that is formed by three polypeptide chains wrapped around a common symmetry axis akin to three interdigitated corkscrews. The folding and biophysical properties of such triple-stranded beta-helices, which are topologically related to amyloid fibers, represent an unsolved biophysical problem. Here, we report structural and biophysical characterization of T4 gp5 beta-helix and its truncated mutants of different lengths. A soluble fragment that forms a dimer of trimers and that could comprise a minimal self-folding unit has been identified. Surprisingly, the hydrophobic core of the beta-helix is small. It is located near the C-terminal end of the beta-helix and contains a centrally positioned and hydrated magnesium ion. A large part of the beta-helix interior comprises a large elongated cavity that binds palmitic, stearic, and oleic acids in an extended conformation suggesting that these molecules might participate in the folding of the complete beta-helix.
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==About this Structure==
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Structure and Biophysical Properties of a Triple-Stranded Beta-Helix Comprising the Central Spike of Bacteriophage T4.,Buth SA, Menin L, Shneider MM, Engel J, Boudko SP, Leiman PG Viruses. 2015 Aug 18;7(8):4676-706. doi: 10.3390/v7082839. PMID:26295253<ref>PMID:26295253</ref>
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[[4jj2]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JJ2 OCA].
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[[Category: Lysozyme]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Boudko, S P.]]
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</div>
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[[Category: Buth, S A.]]
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<div class="pdbe-citations 4jj2" style="background-color:#fffaf0;"></div>
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[[Category: Leiman, P G.]]
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[[Category: Cell-puncturing device]]
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==See Also==
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[[Category: Hydrolase]]
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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[[Category: Intertwined beta-helix]]
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== References ==
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[[Category: Paar-binding]]
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<references/>
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[[Category: T4 gp5 4]]
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__TOC__
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[[Category: Triple beta-helix]]
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</StructureSection>
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[[Category: Escherichia virus T4]]
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[[Category: Large Structures]]
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[[Category: Boudko SP]]
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[[Category: Buth SA]]
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[[Category: Leiman PG]]

Current revision

High resolution structure of a C-terminal fragment of the T4 phage gp5 beta-helix

PDB ID 4jj2

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