4mb4

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{{STRUCTURE_4mb4| PDB=4mb4 | SCENE= }}
 
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===Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag4===
 
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{{ABSTRACT_PUBMED_24598737}}
 
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==About this Structure==
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==Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag4==
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[[4mb4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MB4 OCA].
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<StructureSection load='4mb4' size='340' side='right'caption='[[4mb4]], [[Resolution|resolution]] 1.48&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4mb4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moritella_marina Moritella marina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MB4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.481&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mb4 OCA], [https://pdbe.org/4mb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mb4 RCSB], [https://www.ebi.ac.uk/pdbsum/4mb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mb4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/B1VBB0_MORMI B1VBB0_MORMI]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The four-domain structure of chitinase 60 from Moritella marina (MmChi60) is outstanding in its complexity. Many glycoside hydrolases, such as chitinases and cellulases, have multi-domain structures, but only a few have been solved. The flexibility of the hinge regions between the domains apparently makes these proteins difficult to crystallize. The analysis of an active-site mutant of MmChi60 in an unliganded form and in complex with the substrates NAG4 and NAG5 revealed significant differences in the substrate-binding site compared with the previously determined complexes of most studied chitinases. A SAXS experiment demonstrated that in addition to the elongated state found in the crystal, the protein can adapt other conformations in solution ranging from fully extended to compact.
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==Reference==
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Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.,Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):676-84. doi:, 10.1107/S1399004713032264. Epub 2014 Feb 15. PMID:24598737<ref>PMID:24598737</ref>
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<ref group="xtra">PMID:024598737</ref><references group="xtra"/><references/>
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[[Category: Chitinase]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Malecki, P H.]]
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</div>
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[[Category: Rypniewski, W.]]
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<div class="pdbe-citations 4mb4" style="background-color:#fffaf0;"></div>
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[[Category: Vorgias, C E.]]
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[[Category: Alpha/beta-barrel ig-like]]
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==See Also==
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[[Category: Chbd]]
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*[[Chitinase 3D structures|Chitinase 3D structures]]
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[[Category: Chitin binding domain]]
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== References ==
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[[Category: Chitinase]]
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<references/>
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[[Category: Hydrolase]]
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__TOC__
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[[Category: Hydrolaze]]
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</StructureSection>
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[[Category: Immunoglobulin like domain]]
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[[Category: Large Structures]]
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[[Category: Low activity mutant]]
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[[Category: Moritella marina]]
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[[Category: Nag4]]
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[[Category: Malecki PH]]
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[[Category: Tim-barrel]]
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[[Category: Rypniewski W]]
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[[Category: Vorgias CE]]

Current revision

Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag4

PDB ID 4mb4

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