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9dna

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[[Image:9dna.jpg|left|200px]]<br /><applet load="9dna" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="9dna, resolution 1.800&Aring;" />
 
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'''CRYSTAL STRUCTURE ANALYSIS OF AN A-DNA FRAGMENT AT 1.8 ANGSTROMS RESOLUTION. D(GCCCGGGC)'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE ANALYSIS OF AN A-DNA FRAGMENT AT 1.8 ANGSTROMS RESOLUTION. D(GCCCGGGC)==
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Single crystals of the self-complementary octadeoxyribonucleotide d(GCCCGGGC) have been analysed by X-ray diffraction methods at a resolution of 1.8 A. The tetragonal unit cell of space group P4(3)2(1)2 has dimensions of a = 43.25 A and c = 24.61 A and contains eight strands of the oligonucleotide. The structure was refined by standard crystallographic techniques to an R factor of 17.1% using 1359 3 sigma structure factor observations. Two strands of the oligonucleotide are related by the crystallographic dyad axis to form a DNA helix in the A conformation. The d(GCCCGGGC) helix is characterized by a wide open major groove, a near perpendicular orientation of base pairs to the helix axis and an unusually small average helix twist angle of 31.3 degrees indicating a slightly underwound helix with 11.5 base pairs per turn. Extensive cross-strand stacking between guanine bases at the central cytosine-guanine step is made possible by a number of local conformational adjustments including a fully extended sugar-phosphate backbone of the central guanosine nucleotide.
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<StructureSection load='9dna' size='340' side='right'caption='[[9dna]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[9dna]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9DNA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9DNA FirstGlance]. <br>
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9DNA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9DNA OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9dna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9dna OCA], [https://pdbe.org/9dna PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9dna RCSB], [https://www.ebi.ac.uk/pdbsum/9dna PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9dna ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure analysis of an A-DNA fragment at 1.8 A resolution: d(GCCCGGGC)., Heinemann U, Lauble H, Frank R, Blocker H, Nucleic Acids Res. 1987 Nov 25;15(22):9531-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3684603 3684603]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Heinemann, U.]]
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[[Category: Large Structures]]
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[[Category: a-dna]]
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[[Category: Heinemann U]]
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[[Category: double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:18:31 2008''
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Current revision

CRYSTAL STRUCTURE ANALYSIS OF AN A-DNA FRAGMENT AT 1.8 ANGSTROMS RESOLUTION. D(GCCCGGGC)

PDB ID 9dna

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