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3j6k
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==2.5A structure of lysozyme solved by MicroED== | |
| + | <StructureSection load='3j6k' size='340' side='right'caption='[[3j6k]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3j6k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J6K FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3j4g|3j4g]]</div></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6k OCA], [https://pdbe.org/3j6k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j6k RCSB], [https://www.ebi.ac.uk/pdbsum/3j6k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6k ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad implementation and application of MicroED in structural biology. | ||
| - | + | High-resolution structure determination by continuous-rotation data collection in MicroED.,Nannenga BL, Shi D, Leslie AG, Gonen T Nat Methods. 2014 Aug 3. doi: 10.1038/nmeth.3043. PMID:25086503<ref>PMID:25086503</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 3j6k" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Lysozyme 3D structures|Lysozyme 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Gallus gallus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Lysozyme]] | ||
| + | [[Category: Gonen, T]] | ||
| + | [[Category: Leslie, A G.W]] | ||
| + | [[Category: Nannenga, B L]] | ||
| + | [[Category: Shi, D]] | ||
| + | [[Category: Hydrolase]] | ||
Current revision
2.5A structure of lysozyme solved by MicroED
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