4ov0
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of Bacteriorhdopsin Transferred from Amphipol A8-35 to a Lipidic Mesophase== | |
| + | <StructureSection load='4ov0' size='340' side='right'caption='[[4ov0]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4ov0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum_NRC-1 Halobacterium salinarum NRC-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OV0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ov0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ov0 OCA], [https://pdbe.org/4ov0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ov0 RCSB], [https://www.ebi.ac.uk/pdbsum/4ov0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ov0 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA] Light-driven proton pump. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Amphipols (APols) have become important tools for the stabilization, folding, and in vitro structural and functional studies of membrane proteins (MPs). Direct crystallization of MPs solubilized in APols would be of high importance for structural biology. However, despite considerable efforts, it is still not clear whether MP/APol complexes can form well-ordered crystals suitable for X-ray crystallography. In the present work, we show that an APol-trapped MP can be crystallized in meso. Bacteriorhodopsin (BR) trapped by APol A8-35 was mixed with a lipidic mesophase, and crystallization was induced by adding a precipitant. The crystals diffract beyond 2 A. The structure of BR was solved to 2 A and found to be indistinguishable from previous structures obtained after transfer from detergent solutions. We suggest the proposed protocol of in meso crystallization to be generally applicable to APol-trapped MPs. | ||
| - | + | High-Resolution Structure of a Membrane Protein Transferred from Amphipol to a Lipidic Mesophase.,Polovinkin V, Gushchin I, Sintsov M, Round E, Balandin T, Chervakov P, Schevchenko V, Utrobin P, Popov A, Borshchevskiy V, Mishin A, Kuklin A, Willbold D, Chupin V, Popot JL, Gordeliy V J Membr Biol. 2014 Sep 6. PMID:25192977<ref>PMID:25192977</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4ov0" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Halobacterium salinarum NRC-1]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Balandin T]] | ||
| + | [[Category: Borshchevskiy V]] | ||
| + | [[Category: Chervakov P]] | ||
| + | [[Category: Gordeliy V]] | ||
| + | [[Category: Gushchin I]] | ||
| + | [[Category: Kuklin A]] | ||
| + | [[Category: Mishin A]] | ||
| + | [[Category: Polovinkin V]] | ||
| + | [[Category: Popot JL]] | ||
| + | [[Category: Popov A]] | ||
| + | [[Category: Round E]] | ||
| + | [[Category: Schevchenko V]] | ||
| + | [[Category: Sintsov M]] | ||
| + | [[Category: Utrobin P]] | ||
| + | [[Category: Willbold D]] | ||
Current revision
Structure of Bacteriorhdopsin Transferred from Amphipol A8-35 to a Lipidic Mesophase
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