4pyt

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(New page: '''Unreleased structure''' The entry 4pyt is ON HOLD Authors: Cao, H., Franz, L., Sen, S., Bingman, C., Auldridge, M., Steinmetz, E., Mead, D., Phillips Jr., G.N. Description: Crystal ...)
Current revision (09:49, 22 May 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 4pyt is ON HOLD
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==Crystal structure of a MurB family EP-UDP-N-acetylglucosamine reductase==
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<StructureSection load='4pyt' size='340' side='right'caption='[[4pyt]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4pyt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PYT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PYT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.853&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pyt OCA], [https://pdbe.org/4pyt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pyt RCSB], [https://www.ebi.ac.uk/pdbsum/4pyt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pyt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MURB_BACLD MURB_BACLD] Cell wall formation.[HAMAP-Rule:MF_00037]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We report on the discovery, isolation, and use of a novel yellow fluorescent protein. Lucigen Yellow (LucY) binds one FAD molecule within its core, thus shielding it from water and maintaining its structure so that fluorescence is 10-fold higher than freely soluble FAD. LucY displays excitation and emission spectra characteristic of FAD, with 3 excitation peaks at 276 nm, 377 nm, and 460 nm and a single emission peak at 530 nm. These excitation and emission maxima provide the large Stokes shift beneficial to fluorescence experimentation. LucY belongs to the MurB family of UDP-N-acetylenolpyruvylglucosamine reductases. The high resolution crystal structure shows that in contrast to other structurally resolved MurB enzymes, LucY does not contain a potentially quenching aromatic residue near the FAD isoalloxazine ring, which may explain its increased fluorescence over related proteins. Using E. coli as a system in which to develop LucY as a reporter, we show that it is amenable to circular permutation and use as a reporter of protein-protein interaction. Fragmentation between its distinct domains renders LucY non-fluorescent, but fluorescence can be partially restored by fusion of the fragments to interacting protein domains. Thus, LucY may find application in Protein-fragment Complementation Assays for evaluating protein-protein interactions.
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Authors: Cao, H., Franz, L., Sen, S., Bingman, C., Auldridge, M., Steinmetz, E., Mead, D., Phillips Jr., G.N.
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LucY: A Versatile New Fluorescent Reporter Protein.,Auldridge ME, Cao H, Sen S, Franz LP, Bingman CA, Yennamalli RM, Phillips GN Jr, Mead D, Steinmetz EJ PLoS One. 2015 Apr 23;10(4):e0124272. doi: 10.1371/journal.pone.0124272., eCollection 2015. PMID:25906065<ref>PMID:25906065</ref>
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Description: Crystal Structure of LucY, a MurB family EP-UDP-N-acetylglucosamine reductase
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4pyt" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Unidentified]]
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[[Category: Auldridge M]]
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[[Category: Bingman C]]
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[[Category: Cao H]]
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[[Category: Franz L]]
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[[Category: Mead D]]
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[[Category: Phillips Jr GN]]
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[[Category: Sen S]]
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[[Category: Steinmetz E]]

Current revision

Crystal structure of a MurB family EP-UDP-N-acetylglucosamine reductase

PDB ID 4pyt

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